山羊小热休克蛋白Hspb10基因cDNA克隆及序列分析  

cDNA Cloning and Sequence Analysis of Small Heat Shock Protein 10 Gene of Goat(Capra hircus)

作　　者：施力光[1] 曹婷[1,2] 侯冠彧[1] 周汉林[1] 荀文娟[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,儋州571737 [2]海南大学农学院

出　　处：《中国草食动物科学》2013年第1期5-9,共5页China Herbivore Science

基　　金：中央级公益性科研院所基本科研业务费专项资金(1630032012022)

摘　　要：通过RT-PCR和RACE方法克隆了山羊Hspb10基因cDNA序列并进行了序列分析。结果表明:山羊Hspb10 cDNA全长1 056 bp,编码区全长780 bp,共编码259个氨基酸,提交至GenBank数据库中,收录号为JX067553。用Clustal W方法比对不同物种氨基酸序列同源性,山羊Hspb10与牛的同源性最高,核苷酸和氨基酸序列相似性分别为93.4%和96.5%;编码氨基酸序列BLAST比对结果也表明,哺乳动物Hspb10氨基酸序列极为保守,与禽类差异较大。获得山羊Hspb10 cDNA序列,可以为分子水平上研究山羊Hspb10的生物学功能奠定基础。Full length cDNA of goat Hspb 10 gene was cloned by RT-PCR and rapid amplification of cDNA ends （RACE） and the sequences were analyzed.The results showed that cDNA sequence from goat Hspb 10 was 1 056 bp, including 780 bp in CDS which encoded a protein of 259 amino acids; the sequence was submitted to GenBank and the Accession No. was JX067553;the highest identity of goat Hspb 10 nucleotide sequence and amino acid sequence were 93.4% and 96.5%, compared with cattle by using Clustal W alignment;the amino acid sequences were conservative among the mammals,whereas there was a great difference with poultry by BLAST. The obtained goat Hspb 10 cDNA sequence will make a basis for further study of its biological function on a molecular level.

关 键 词：山羊 Hspb10基因 CDNA克隆 序列分析 

分 类 号：S827.2[农业科学—畜牧学]