辽宁绒山羊UMP/CMP激酶基因的表达及分析  

Expression Analysis of UMP/CMP Kinase Gene in the Liaoning New Breed Cashmere Goat

作　　者：金梅[1] 张亭亭[1] 王艳杰[1] 曲杨乐[1] 徐明[1] 李秀娜[1] 

机构地区：[1]辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室,大连116029

出　　处：《中国草食动物科学》2013年第1期10-14,共5页China Herbivore Science

基　　金：国家"十一五"科技支撑计划(2009BADA5B01);国家自然科学基金(30972079);大连市科技计划项目(2010B14NC104);辽宁省百千万人才基金

摘　　要：在前期构建辽宁绒山羊皮肤cDNA文库的基础上,测通得到了新基因UMP/CMP激酶的全长序列,并对UMP/CMP激酶基因进行生物信息学分析。然后,通过RT-PCR检测该基因在心、肝、脾、肺及肾中的表达。生物信息学分析显示,UMP/CMP激酶基因全长1 081 bp,能够编码含有228个氨基酸的蛋白质,亮氨酸、甘氨酸、赖氨酸含量较高。UMP/CMP激酶含有较高比例的无规卷曲结构(51.75%),并发现其含有UMP/CMP激酶蛋白结构域。蛋白三级结构、进化树分析显示,辽宁绒山羊和牛的UMP/CMP激酶基因的同源性最高。UMP/CMP激酶基因存在跨膜结构域,并且存在信号肽剪切位点。该基因中有8个Ser,2个Thr,2个Tyr可能成为蛋白激酶磷酸化位点。RT-PCR检测显示,UMP/CMP激酶基因特异地表达于皮肤。这些研究结果为深入研究辽宁绒山羊的种质遗传特性奠定了基础。In order to study the genetic characteristics of Liaoning newbreed cashmere goat,on the basis of construction of a skin cDNA library and isolation of a full-length cDNA clone termed UMP/CMPK,the bioinformatics analysis and semi- quantitative RT-PCR were used to detect the expression of the UMP/CMPK gene in all internal organs. A bioinformatics analysis showed that the UMP/CMPK gene encoded a putative 228 amino acid protein that exhibited a high content of leucine, glycine and lysine and had a lower content of histidine and tryptophan. The secondary structure prediction revealed a high proportion of random coils （51.75%）. UMP/CMPK contained a conservative UMP/CMP kinase protein structure domain. UMP/CMPK of the goat and cattle had the highest homology. UMP/CMPK also had transmembrane domain and signal peptide. 8 Ser,2 Thr and 2 Tyr may be phosphorylation sites in CMP/UMPK protein.RT-PCR analysis showed that UMP/ CMPK were only present in goat skin. This research provides an experimental basis for the further study of the genetic characteristics of the goat.

关 键 词：辽宁绒山羊 UMP CMP激酶基因 生物信息学分析 RT—PCR 

分 类 号：S827.2[农业科学—畜牧学]