肺腺癌组织中HSP70多肽复合物的分离及纯化  

Separation and purification of heat shock protein 70 peptide complex from human lung adenocarcinoma tissue

作　　者：李杰[1] 梁守沛[2] 陆皓泉[1] 孙鹏辉[1] 王小龙[1] 许东[1] 姚武[1] 周舫[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,郑州450001 [2]郑州人民医院医务科,郑州450003

出　　处：《郑州大学学报（医学版）》2012年第6期753-755,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30600490;河南省教育厅科技创新人才支持计划基金资助项目2008HASTIT028;河南省教育厅自然科学研究基金资助项目2008A320018

摘　　要：目的:探讨肺腺癌组织中HSP70多肽复合物分离及纯化的可行性方案。方法:采用裂解液裂解、超声破碎、ConA-sepharose亲和层析和DEAE阴离子交换层析方法,从肺腺癌组织中分离纯化HSP70蛋白复合物;通过SDS-PAGE电泳、Westernblot检测获得蛋白;运用Bradford法对其进行定量分析。结果:通过该实验方法可以从1g肺癌组织中获取110μg左右的HSP70多肽复合物,其相对分子质量为70000。结论:采用ConA-sepharose亲和层析和DEAE阴离子亲和层析相结合的蛋白纯化法可以获取高纯度的HSP70多肽复合物。Aim：To explore a method of separating and purifying HSP70 peptide complex from human lung adenocarcinoma.Methods：HSP70 peptide complex was separated and purified from human lung adenocarcinoma tissue by means of cell lysis,ultrasonication,ConA-sepharose column,and DEAE ion-exchange chromatogaraphy.The purified fractions were tested by SDS-PAGE and Western blot.And quantitative analysis was made by Bradford.Results：About 110 μg HSP70 peptide complex was obtained from 1 g lung adenocarcinoma tissue by this experimental method,with relative molecular weight of 70 000.Conclusion：The high purity HSP70 peptide complex could be obtained by ConA-sepharose column and DEAE ion-exchange chromatogaraphy.

关 键 词：肺腺癌 HSP70多肽复合物 蛋白分离纯化 

分 类 号：R135.99[医药卫生—劳动卫生]