左卡尼汀对肾缺血再灌注损伤大鼠肾组织MPO和NO含量的影响  

Effects of L-carnitine on the contents of MPO and NO in rats with renal ischemic reperfusion injury

作　　者：许益笑[1,2] 张睿喆[3] 王德选[2] 倪世容[2] 王万铁[2] 郑绿珍[2] 王丽[4] 熊锡山[4] 郭坤元[1] 

机构地区：[1]南方医科大学珠江医院血液科,广州510282 [2]温州医学院病理生理学教研室,温州325000 [3]郑州大学基础医学院,郑州450001 [4]上海长征医院肾脏病研究所,上海200030

出　　处：《郑州大学学报（医学版）》2012年第6期810-813,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨左卡尼汀对肾缺血再灌注损伤(RIRI)大鼠能量代谢的影响及其机制。方法:健康SD大鼠48只,随机分为对照组和左卡尼汀组,每组24只。2组大鼠均建立RIRI模型,左卡尼汀治疗组大鼠缺血前15min及再灌注(IR)后40min尾静脉注射左卡尼汀500mg/kg,2组分别于IR0、1、6和12h各处死6只大鼠。检测各组大鼠血清尿素氮(BUN)和肌酐(Cr)、肾组织髓过氧化物酶(MPO)和一氧化氮(NO)含量,并观察肾组织病理变化。结果:2组IR0、1、6、12h大鼠血清BUN和Cr比较,差异有统计学意义(F组间=5.116和6.602,F时间=15.235和47.118,P均<0.05),2组IR6、12h较IR0h升高,左卡尼汀组IR6、12h较对照组下降(P<0.05)。2组IR0、1、6、12h大鼠肾组织MPO和NO含量比较,差异有统计学意义(F组间=6.259和3.729,F时间=7.709和39.671,P均<0.05)。2组IR12h大鼠肾组织MPO含量较IR0h升高,左卡尼汀组IR12h大鼠肾组织MPO含量低于对照组(P<0.05);2组IR12h大鼠肾组织NO含量较IR0h降低,左卡尼汀组IR12h大鼠肾组织NO含量高于对照组(P<0.05)。左卡尼汀组IR6和12h大鼠肾小管损伤明显减轻。结论:左卡尼汀对肾缺血再灌注损伤具有保护作用,其机制与减少中性粒细胞聚集,降低肾组织MPO释放,增加NO合成有关。Aim：To investigate possible mechanisms involved in the protection of L-carnitine against renal ischemic reperfusion injury（RIRI） in rats.Methods：A total of 48 SD healthy rats were divided into control and L-carnitine groups（n=24）.Model of RIRI was established in the two groups,and 6 rats were killed at ischemic reperfusion（IR） 0,1,6,and 12 h.The contents of serum urea nitrogen（BUN） and creatinine（Cr）,the myeloperoxidase（MPO） and NO in nephridial tissue were measured.The pathological changes in renal tissue were observed.Results：In the two groups,Cr and BUN contents of the IR 0,1,6,12 h rats were obviously different（Fgroup=5.116 and 6.602,Ftime=15.235 and 47.118,P0.05）.Comparing with those of IR 0 h killed rats,Cr and BUN contents of the IR 6 and 12 h killed rats increased significantly;Cr and BUN contents of the IR 6 and 12 h killed rats were lower in L-carnitine group than those in the control group（P0.05）.In the two groups,MPO and NO contents of the IR 0,1,6,12 h rats were obviously different（Fgroup=6.259 and 3.729,Ftime=7.709 and 39.671,P0.05）.Comparing with those of IR 0 h killed rats,MPO content of the IR 12 h killed rats increased significantly in the two groups;MPO content of the IR 12 h killed rats was higher in control group than that in the L-carnitine group（P0.05）.In the two groups,comparing with those of IR 0 h killed rats,NO content of the IR 12 h killed rats decreased significantly;NO content of the IR 12 h killed rats was lower in control group than that in the L-carnitine group（P0.05）.In addition,renal histological injuries were attenuated after L-carnitine administration.Conclusion：L-carnitine protects kidney against acute RIRI through inhibiting neutrophil aggregation,lowering the release of MPO and increasing the synthesis of NO in the renal tissue.

关 键 词：左卡尼汀 肾脏 缺血再灌注损伤 髓过氧化物酶 一氧化氮 大鼠 

分 类 号：R692.5[医药卫生—泌尿科学]