柿叶提取物对CCl_4致小鼠急性肝损伤保护作用的研究  

Protective effects of extract from persimmon leaves on tetrachloride-induced hepatotoxicity in mice

作　　者：张艳[1] 杨建雄[1] 马蕊[1] 王芳[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2013年第1期65-68,共4页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20175012)

摘　　要：探讨了柿叶醇提物的乙酸乙酯萃取制剂(EAF)对小鼠急性肝损伤的保护作用.腹腔注射四氯化碳(CCl4)致小鼠急性肝损伤模型,测定不同剂量的EAF对血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)活性,肝超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量的影响,同时对肝组织进行病理学检查.结果表明,EAF具有剂量依赖性地降低血清中ALT、AST、AKP值,增强肝中SOD、GPx、CAT的活性,降低肝组织匀浆中MDA的含量,减轻CCl4对肝脏细胞的病理损伤.100mg/kg是EAF对肝损伤的合适保护剂量.适量的EAF对CCl4所致小鼠急性肝损伤有较好的保护作用.The effects of ethyl acetate fraction （EAF） containing substances ethanol extracted from persimmon leaves on carbon tetrachloride-induced hepatotoxicity in mice were inrestigated. The acute hepatic injury model was carried out by CC14 injected intraperitoneally. Then, the therapeutic effects of EAF were evaluated based on the activities of alanine aminotransferas （ALT）, aspartate aminotransferase （AST）, alkaline phosphatase （AKP）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, catalase （CAT） and the content of malondialdehyde （MDA） in liver, and the changes of hepatic pathohistology. The results indicated that different doses of EAF significantly lowered ALT, AST, AKP levels in serum and MDA level in liver along with the restoring of hepatic enzymes like SOD, GPx, and CAT against CCl4 treated mice. The pathological changes were also significantly improved in the mice of treating group. Furthermore, it was found that 100 mg/kg was the optimal dose for exhibiting the greatest hepatoprotective effects on CCl4-induced liver injury, indicating that EAF of adequate dose exhibits potent hepatoprotective effects on CCl4-induced acute liver damages in mice.

关 键 词：柿叶 四氯化碳 急性肝损伤 小鼠 

分 类 号：R284.2[医药卫生—中药学]