鲍曼不动杆菌携带消毒剂耐受相关基因的研究  

Analysis of Antiseptics Resistance-Relative Genes in Acinetobacter Baumannii

作　　者：林健濂[1] 伍晓锋[2] 邓思健[1] 潘海燕[1] 钟海波[1] 曾瑜[1] 邹石海[1] 赖艳榕[1] 范秀[1] 汪得喜[1] 

机构地区：[1]广州市红十字会医院,广东广州510220 [2]广州医学院第一附属医院广州呼吸疾病研究所,广东广州510120

出　　处：《今日药学》2012年第12期712-715,共4页Pharmacy Today

基　　金：广东省医学科研基金项目(编号:2012A2012502);广州市卫生局医药卫生科技项目(编号:20121A011049)

摘　　要：目的研究鲍曼不动杆菌所携带的Ⅰ型整合子与耐消毒剂基因。方法琼脂扩散法检测菌株对7种抗菌药物的药敏结果;用聚合酶链反应(PCR)方法检测Int-1、qacA/B、qacEΔ1-sul1、qacG、smr、Isabal和tnp513共7种与消毒剂耐受相关基因。结果 24株菌对亚胺培南、哌拉西林/他唑巴坦、头孢他啶、四环素、头孢哌酮/舒巴坦、甲氧苄啶/磺胺甲噁唑、阿米卡星等抗生素敏感率分别为12.5%、4.2%、4.2%、0、12.5%、8.3%和8.3%;PCR结果显示,在24株菌中,qacEΔ1-sul1、Isabal和Int-1基因阳性率分别为79.2%、83.3%和33.3%。qacA/B、qacG、tnp513和smr4种基因检测结果为阴性。结论本组鲍曼不动杆菌对广谱抗生素耐药严重,并携带了Ⅰ型整合子和消毒剂耐药基因。应规范此类抗生素的使用并采取相应的消毒措施防止多重耐药菌株在院内传播。Objective To investigate the prevalence of integron Ⅰ and antiseptics resistance genes in Acinetobacter baumannii strains.Methods Susceptibilities of 7 broad-spectrum antibiotics against A.baumannii isolates was determined by agar dilution according to the CLSI standard,and antiseptics resistance genes such as Int-1,qacA / B,qacEΔ1-sul1,qacG,smr,Isabal,and tnp513 were detected by PCR.Results The susceptibilities to imipenem,piperacillin-tazobactam,ceftazidime,tetracycline,cefoperazone-sulbactam,trimethoprom-sulfamethoxazole,amikacin were 12.5%,4.2%,4.2%,0,12.5%,8.3%,and 8.3%,respectively.The gene prevalence of qacEΔ1-sul1,Isabal,and Int-1 genes were 79.2%,83.3%,and 33.3%,respectively,while other 4 genes were not detected.Conclusion Acinetobacter baumannii strains we studied have resistances to broad-spectrum antibiotics seriously and carried Int-1 and antiseptics resistance genes.Accordingly,broad-spectrum antibiotics should be standardized utilized and effective disinfection measures should be carried out to prevent the dissemination of multidrug resistant strains in the hospital.

关 键 词：鲍曼不动杆菌 Ⅰ型整合子 消毒剂 耐药基因 

分 类 号：R969[医药卫生—药理学]