中国维族人群DNA酶Ⅰ基因多态性与急性心肌梗死的相关性研究  

Study on Correlation of Enzyme Ⅰ Gene Polymorphism with Acute Myocardial Infarction in Uygur Nationality

作　　者：许翔[1] 李莉[1] 高玮[1] 李佳[1] 

机构地区：[1]上海交通大学附属第一人民医院检验科,上海200080

出　　处：《现代检验医学杂志》2012年第6期19-22,共4页Journal of Modern Laboratory Medicine

基　　金：上海市科委西部合作项目《中国汉族及维吾尔族人群DNA酶Ⅰ基因多态性特点与急性心机梗死的关系》,项目编号：08495801900.

摘　　要：目的探讨DNA酶I（DNaseI）基因多态性在急性心肌梗死（acutemyocardialinfarction，AMI）患者中的分布及其与患者血脂、血糖水平的关系。方法选取急性心肌梗死患者157例及同期健康体检者148例，提取外周血基因组DNA．通过高温连接酶检测法（PCR-LDR）检测DNA酶Ⅰ基因A2317G（rsl053874）位点基因型。酶法检测血清总胆固醇、三酰甘油及空腹血糖水平；选择性遮蔽法测定低密度脂蛋白胆固醇和高密度脂蛋白胆固醇水平。结果A2317G位点3组基因型（AA／AG／GG）及两种等位基因（A2317，G2317）在对照组与AMI组分布频率差异均有统计学显著性意义（P值分别为0．025，0．0095）；AMI组3种基因型患者血清总胆固醇（P=0．799）、三酰甘油（P=0．78）、高密度脂蛋白胆固醇（P＝0．815）、低密度脂蛋白胆固醇（P＝0．576）水平差异均无统计学显著性意义，空腹血糖水平差异有统计学意义（P＝0．043）。结论DNA酶I基因多态性与急性心肌梗死易感性有一定相关性，与患者血脂无关，与空腹血糖水平有相关性。Objective To investigate the frequency of DNA enzyme Ⅰ gene polymorphism in Uygurs with acute myocardial infarction and its＇ association with blood glucose and lipid. Methods 157 cases of acute myocardial infarction patients and 148 cases of healthy controls were selected during the same period. Blood samples of all the 305 Uygurs in Xinjiang were col- lected. Genomic DNA were extracted from all blood samples, PCR-LDR reaction was employed to detect the A2317G （rs1053874） mutation of Deoxyribonuclease I gene,genotype and allele distribution were analyzed. Meantime,blood glucose and lipid were measured in fasting state by biochemical methods. Results The distribution frequencies of both the genotypes and alleles were significantly different between the control and AMI group. Genotypes of A2317G were not associated with serum total cholesterol,triglyceride,high-density lipoprotein cholesterol and low density lipoprotein-cholesterol （P〉0.05）, but were closely related with fasting glucose （P〈0.05）. Conclusion Tl^e polymorphism of DNA enzyme Ⅰ gene had a cer- tain relationship with the susceptibility of acute myocardial infarction in Uygurs in Xinjiang. Genotypes of A2317G were not associated with blood lipid,but were closely related with fasting glucose.

关 键 词：脱氧核糖核酸酶Ⅰ 基因多态性 急性心肌梗死 血糖 血脂 

分 类 号：R542.22[医药卫生—心血管疾病] Q786[医药卫生—内科学]