拭样增菌对实时荧光PCR法检测孕妇B族链球菌感染的影响  

Effect of Swabs Enrichment on the Detection of Group B-Streptococcal Infection in Gravid Women by Real-time Fluorescent PCR

作　　者：边佳明[1] 丁媛媛[1] 杨凡[1] 张梅[1] 许景峰[1] 

机构地区：[1]北京军区总医院药理科临床基因扩增检验室,北京100700

出　　处：《现代检验医学杂志》2012年第6期102-104,共3页Journal of Modern Laboratory Medicine

摘　　要：目的比较拭样增茵或不增茵对实时荧光PCR法检测孕妇产道B族链球菌感染率的影响。方法收集临床孕35～37周妇女阴道直肠拭子1000例，平均分为增茵检测组和未增茵检测组。增茵组拭子置于增菌液室温过夜增菌后40C保存待检，未增茵组拭子直接4℃保存待检，所有PCR检测在临床采样后48h内完成，检验指标为B族链球菌的阳性检出率。结果X2检验结果显示，拭样增茵显著影响实时荧光PCR法对B族链球菌的阳性检出率，增菌后B族链球茵阳性检出率提高了1．7倍（P=0．006）。结论提取前对临床标本适当增茵在临床PCR检验实验室具有可操作性，更好地避免了假阴性的出现，提升了B族链球菌产前筛查的意义。Objective To compare the effect of swabs enrichment on the detection of group B-streptococcal （GBS） infection by real-time fluorescent PCR in birth canal of gravid women. Methods 1 000 vagina-rectum swabs at 35～37 week＇s gesta- tion were collected and evenly divided into enrichment group and non-enrichment group. Swabs of enrichment group were en- riched in sterile broth overnight at room temperature followed by storage at 4～3 ready for detection,while swabs of non-en- richment group were stored at 4℃ ready for detection. All PCR detections must be completed within 48h to statistic GBS i- solating rate. Results Chisquare test showed that enrichment before DNA extraction remarkably affected isolating rate of GBS by real-time fluorescent PCR,and enrichment group had a higher isolating rate （1.7 times of control,P=0. 006） than non-enrichment group. Conclusion The proper enrichment for clinical specimens is very feasible in clinical gene amplifica- tion test lab,by which false-negative was greatlyavoided,improving the value of GES screen before delivery

关 键 词：实时荧光PCR B族链球菌 围产期 

分 类 号：R378.12[医药卫生—病原生物学] Q503[医药卫生—基础医学]