模拟失重抑制荷黑素瘤小鼠的T淋巴细胞抗肿瘤免疫功能  

Simulated weightlessness inhibits antitumor immunity of T lymphocytes in melanoma-bearing mice

作　　者：司少艳[1] 宋淑军[1] 史亮[2] 刘俊丽[1] 徐冰心[3] 易勇[4] 谭小青[1] 张建中[1] 

机构地区：[1]解放军第306医院病理实验科,北京100101 [2]解放军第306医院口腔科,北京100101 [3]解放军第306医院特种病科,北京100101 [4]解放军第306医院检验科,北京100101

出　　处：《细胞与分子免疫学杂志》2013年第2期127-131,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的研究模拟失重对小鼠T淋巴细胞抗肿瘤免疫功能的影响。方法小鼠右后肢皮下注射B16细胞建立移植性恶性黑素瘤模型,采用头低位-15°～20°尾吊小鼠模拟失重模型。观察模拟失重对荷瘤小鼠肿瘤体积和生存时间的影响;采用全自动血细胞分析仪和流式细胞仪分别检测模拟失重条件下荷瘤小鼠外周血白细胞、淋巴细胞的变化和T淋巴细胞亚群的变化;采用ELISA法和LDH释放法分别检测模拟失重对肿瘤细胞诱导T淋巴细胞产生IL-2、TNF-α、IFN-γ的水平和肿瘤特异性CTL对肿瘤细胞杀伤活性的影响。结果与对照组相比,模拟失重组荷瘤小鼠肿瘤生长速度加快,生存期缩短,外周血淋巴细胞总数降低,淋巴细胞比例降低,CD3+、CD4+/CD3+、CD8+/CD3+T淋巴细胞所占比例降低,丝裂原诱导的脾脏T淋巴细胞增殖能力降低(P<0.05或P<0.01)。模拟失重条件下,肿瘤细胞诱导产生的细胞因子IL-2、IFN-γ和TNF-α水平降低,肿瘤特异性CTL对肿瘤细胞的杀伤活性降低(P<0.05或P<0.01)。结论模拟失重抑制小鼠T淋巴细胞抗肿瘤免疫功能。Objective To investigate the effects of simulated weightlessness on antitumor immunity of T lymphocytes in mice.Methods The malignant melanoma was xenografted by subcutaneous injection of B16 cells into the right hind limb of every mouse.The mice suspended by tail at a-15° to 20° head-down tilt were used as simulated weightlessness models.The effects of simulated weightlessness on tumor volume and survival time were observed.Tthe numbers of leucocytes,lymphocytes and T lymphocyte subsets in peripheral blood of tumor-bearing mice under simulated weightlessness were monitored by an automatic hemacytometer and a flow cytometer.The effects of simulated weightlessness on the production of IL-2,TNF-α and IFN-γ in T lymphocytes and the cytotoxicities of tumor-specific CTLs to tumor cells were analyzed by ELISA and LDH release.Results Compared with control group,the tumors grew faster,the survival times were shorter,the number of lymphocytes,the ratio of lymphocytes,CD3＋,CD4＋/CD3＋ and CD8＋/CD3＋ T lymphocytes in peripheral blood droped,and the proliferation of splenic T lymphocytes induced by mitogen was reduced（P0.05 or P0.01） in the simulated weightlessness group.The production of IL-2,TNF-α and IFN-γ induced by tumor cells and cytotoxicities of tumor-specific CTLs to tumor cells were inhibited in mice under simulated weightlessness（P0.05 or P0.01）.Conclusion Simulated weightlessness inhibits antitumor immunity of T lymphocytes.

关 键 词：模拟失重 恶性黑素瘤 T淋巴细胞 抗肿瘤免疫 

分 类 号：R392.12[医药卫生—免疫学] R852.22[医药卫生—基础医学]