葡萄膜炎患者外周血TCR Vα谱系多态性分析  

Lineage polymorphism of TCR Vα CDR3 in peripheral blood of uveitis patients

作　　者：邹红云[1] 余伍忠[1] 黄红艳[1] 郭琨[2] 张璐[1] 魏克娜[1] 焦敏[1] 卢静[1] 张琼[1] 

机构地区：[1]兰州军区乌鲁木齐总医院,新疆乌鲁木齐830000 [2]解放军第273医院,新疆库尔勒841000

出　　处：《细胞与分子免疫学杂志》2013年第2期182-185,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81172840);兰州军区医药卫生科研基金资助项目(CWS10JA21)

摘　　要：目的探讨葡萄膜炎患者外周血T细胞受体α链可变区互补决定区3(TCR VαCDR3)谱系特点及多态性。方法采用RT-PCR扩增葡萄膜炎患者外周血单个核细胞(PBMC)中TCR Vα34个亚家族,经免疫扫描谱型技术分析34个亚家族谱型。结果 5例正常健康人PBMC TCR VαCDR3谱型多数呈高斯分布,4例葡萄膜炎患者外周血T细胞均出现多个TCR Vα亚家族谱型的异常改变,异常峰型包括单峰、寡峰/寡峰趋势,偏峰和不规则异常峰型。34个TCR Vα亚家族中,不同亚家族异常峰型出现的频率不同,非正态异常峰型出现频率较高的亚家族有Vα13和Vα17(均为3/4),而Vα1.1、Vα10、Vα20、Vα28和Vα28亚家族均未出现异常峰型。TCR Vα7在HLA-B27阴性的患者(U2)中出现异常峰型,在HLA-B27阳性的3个患者(U1、U3、U4)中并未出现这个亚家族的异常;TCR Vα13则相反,即在HLA-B27阳性的3个患者中均出现异常峰型,而在HLA-B27阴性的患者中正常。结论葡萄膜炎患者PBMC TCR VαCDR3谱系具有显著多态性特点,单/寡克隆增生的T细胞有可能是葡萄膜炎发病中的自身反应性T细胞。Objective To study the lineage polymorphism of TCR Vα complementarity determining region 3（CDR3） in the peripheral blood of uveitis patients.Methods Thirty-four subfamilies of TCR Vα CDR3 in the peripheral blood mononuclear cells（PBMC） of uveitis patients were amplified by RT-PCR,and then TCR Vα CDR3 lineage polymorphism was analyzed by immunoscope spectratyping.Results Most spectral type of the 34 subfamilies in 5 normal controls showed Gauss distribution.TCR Vα CDR3 scanning spectrum of 4 uveitis patients showed abnormal distribution peak,including monoclonal,oligoclonal/oligoclonal trend,skewing peak and irregular abnormal peak.The frequencies of abnormal peak type varied in the 34 TCR Vα subfamilies.A higher frequency of abnormal peak type was found in Vα13 and Vα17 subfamilies,while no abnormal peak type was found in Vα1.1,Vα10,Vα20 and Vα28 subfamilies.Abnormal peak was shown in Vα7 subfamily in the HLA-B27-negative patient（U2）,but no abnormal peak was shown in Vα7 subfamily in the 3 HLA-B27-positive patients（U1,U3,U4）;Conversely,abnormal peak type of TCR Vα13 was found in all the 3 HLA-B27-positive patients,but normal in HLA-B27-negative patients.Conclusion TCR Vα CDR3 lineage has significant characteristic polymorphism in the peripheral blood of uveitis patients.Monoclonal/oligoclonal expansion of T cells may be autoreactive T cells in nature and they may be involved in pathogenesis of uveitis.

关 键 词：葡萄膜炎 T细胞受体 Vα亚家族 互补决定区3 免疫扫描谱型分析 

分 类 号：R392.12[医药卫生—免疫学] R392.33[医药卫生—基础医学]