环磷酰胺对肾病综合征患儿淋巴细胞P-gp170表达的影响  

Effect of cyclophosphamide on expression of lymphocyte P-gp170 in children with nephrotic syndrome

作　　者：谢海涛[1] 关凤军[1] 董晨[1] 高莉莉[1] 杨峰[1] 王军[1] 

机构地区：[1]徐州医学院附属医院儿科,江苏省221000

出　　处：《江苏医药》2013年第2期181-184,共4页Jiangsu Medical Journal

摘　　要：目的探讨环磷酰胺(CTX)对肾病综合征(NS)患儿P-糖蛋白170(P-gp170)表达的影响。方法以流式细胞术检测频繁复发NS患儿(33例,研究组)CTX治疗前后外周血CD3淋巴细胞P-gp170表达,反相高效液相色谱法检测血清CTX浓度。对照组为14例糖皮质激素(GC)敏感非依赖NS患儿。结果研究组治疗前CD3/P-gp170表达为(8.25±2.03)%,明显高于治疗后3个月的(2.92±1.94)%(P<0.01);治疗后CD3/P-gp170表达与血清CTX浓度呈负相关(P<0.05)。结论下调外周血淋巴细胞P-gp170的表达可能为CTX逆转NS患儿GC耐药的机制之一。CTX可通过抑制P-gp170表达而节约GC用量。Objective To investigate the changes of P-glycoprotein 170（P-gp170） expression in active peripheral lymphocytes（CD3） in children with nephrotic syndrome（NS） during cyclophosphamide（CTX） treatment. Methods P-gp170 expression in peripheral blood and serum concentration of CTX were determined with flow cytometry and RP-HPLC,respectively,in 33 children with frequently and repeatedly relapsed NS（group A）.The control group（C） consisted of 14 NS children,who was sensitive to steroid without glucocorticoid dependence. Results The expression of P-gp170 was lower after CTX therapy for 3 months than that before[（2.92±1.94）% vs.（8.25±2.03）%]（P〈0.01）.The expression of P-gp170 after CTX therapy was negatively correlated with serum concentration of CTX（P〈0.05）. Conclussion Down-regulation of P-gp170 expression in CD3 may be one of the mechanisms for CTX reversing NS children with glucocorticoid-resistance.CTX may reduce the requirement for glucocorticoid by inhibiting P-gp170 expression in CD3 in NS children.

关 键 词：环磷酰胺 肾病综合征 P-糖蛋白170 儿童 

分 类 号：R969[医药卫生—药理学]