特发性膜性肾病与M型磷脂酶A2受体基因多态性的相关性  

Correlation of M-type phospholipase A2 receptor genetic polymorphism with idiopathic membranous nephropathy

作　　者：周广宇[1] 孙延霞[2] 周立祥[3] 于晶[1] 郭莹[1] 尹敏[1] 

机构地区：[1]吉林大学中日联谊医院肾病内科,长春130033 [2]吉林大学中日联谊医院血液科,长春130033 [3]吉林大学第一医院神经创伤外科

出　　处：《中华肾脏病杂志》2013年第1期1-5,共5页Chinese Journal of Nephrology

基　　金：吉林省科技厅国际科技合作项目（20100738）;吉林大学基本科研业务费项目--科学前沿与交叉学科创新项目（2011.01.2012.12）;吉林大学与日本北里大学校际交流项目（2011.01.2012.01）

摘　　要：目的研究M型磷脂酶A2受体（PLA2R）的基因多态性与中国东北汉族人群特发性膜性肾病（IMN）的相关性。方法应用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）技术检测95例IMN患者（IMN组）及232例健康体检者（HC组）PLA2R基因rs35771982和rs3828323两个位点的基因型和等位基因频率。结果IMN组和HC组间性别、体质量指数（BMI）相匹配。IMN组的平均年龄、Scr、总胆固醇（TC）和24h尿蛋白量均显著高于HC组（均P〈0．01）；血清Alb和eGFR显著低于HC组（P〈0．01）。IMN组rs35771982位点CC基因型和C等位基因的频率均显著高于HC组（χ2=13．658，P=0．001；χ2=15．315，P=9．10×10^-5）。而两组间rs3828323位点基因型和等位基因频率的差异无统计学意义。rs35771982位点CC基因型与年龄、性别、BMI、血压、血清Alb、TC、Scr、eGFR及24h尿蛋白量等指标无相关性。rs35771982位点基因型、年龄、TC、Scr及eGFR与IMN的发病相关，rs35771982位点的CC基因型是IMN的危险因素（OR=4．408，95％CI 1．488～13．058）。结论中国汉族人群PLA2Rrs35771982位点基因多态性可能与IMN易感性相关，而rs3828323位点基因多态性与IMN无相关。rs35771982位点CC基因型是IMN的危险因素。Objective To investigate the correlation of M- type phospholipase A2 receptor （PLA2R） genetic polymorphism in two single nucleotide polymorphisms （SNPs） with idiopathic membranous nephropathy （IMN） of Chinese Han population in Northeast China. Methods A total of 327 individuals were enrolled in the study including 95 adult patients with biopsy-proved IMN （IMN group） followed up for （25.4± 11.6） months and 232 healthy people identified by healthy examination in China- Japan Union Hospital of Jilin University （HC group）. Polymerase chain reaction- restriction fragment length polymorphism （PCR- RFLP） was used to detect the genotype and allele frequency of rs35771982 and rs3828323 site in PLA2R gene. The χ2 test was performed to compare the distribution difference of allelic frequency and genotype frequency of the two sites in PLA2R gene between two groups. Unconditional Logistic regression analysis was used to determine the risk factor of IMN. Results IMN and HC group were matched in male predominance and body mass index （BMI）. Patients with IMN were older than the healthy controls and had higher Set, serum total cholesterol （TC）, 24-hour urine protein level and lower serum albumin （Alb） level, lower estimated glomerular filtrationrate （eGFR） than the healthy controls （all P 〈 0.01）. The CC genotype frequency and the C allele frequency at SNP rs35771982 site of PLA2R gene in IMN group were significantly higher than those in HC group （χ2= 13.658, P=0.001; χ2= 15.315, P=9.10× 10^-5）, whereas there was no distribution difference of genotype and allele frequency at rs3828323 site between two groups （χ2= 2.844, P = 0.241;χ2= 2.959, P= 0.085）. The CC genotype at rs35771982 site in patients with IMN was not related to, age, gender, BMI, blood pressure and several laboratory indexes such as Alb, TC, Scr, eGFR and 24-hour urine protein level （all P 〉 0.05）. Unconditional Logistic regression analysis revealed that the genotype at rs35771982, age, TC, Scr a

关 键 词：肾小球肾炎 膜性 受体 M型磷脂酶A2 多态性 单核苷酸 

分 类 号：R692.3[医药卫生—泌尿科学]