肝、肾移植受者外周血基因表达差异分析  

Differences in peripheral blood gene expression profiles between liver and kidney transplantation recipients

作　　者：徐洪来[1] 肖敏[2] 杨超[1] 高义[1] 雒真龙[1] 周鸿敏[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,湖北武汉430030 [2]华中科技大学同济医学院附属协和医院皮肤科,湖北武汉430022

出　　处：《南方医科大学学报》2013年第2期166-171,共6页Journal of Southern Medical University

基　　金：国家重点基础研究发展计划973计划(2009CB522407)~~

摘　　要：目的探讨肝移植与肾移植受者外周血免疫细胞基因表达差异。方法从GEO数据库获取27个肝移植受者及25个肾移植受者外周血的基因芯片表达谱数据。应用GSEA软件进行基因集富集分析,应用Cytoscape软件对差异基因进行生物网络分析,获得肝、肾移植相关核心基因。结果 GSEA分析筛选出肝、肾移植领头亚群高重叠基因各20个。差异基因生物网络分析,筛选出肝、肾移植相关中心蛋白质(基因)数目分别为14及13;结合GSEA及生物网络分析结果,获得核心基因各5个。结论外周血免疫细胞的转录、翻译调节机制在肝移植免疫调节中发挥了更重要的作用,而在肾移植信号蛋白质的相互作用网络发挥了主要的免疫调控功能;免疫细胞的能量代谢与免疫调控存在密切关系;外周血肝、肾移植核心基因发挥了重要的免疫调节功能。Objective To investigate the differences in the gene expression profiles of the peripheral blood immune ceils between liver and kidney transplantation recipients. Methods A dataset containing the gene expression profiles of 27 liver transplantation recipients and 25 kidney transplantation recipients （from GSE22229 and GSE28842, respectively） was downloaded from the GEO database. By combining gene set enrichment analysis （GSEA） and biological network analysis of the differentially expressed genes using Cytoscape software, we analyzed the core genes closely related to liver or kidney transplantations. Results GSEA identified 20 highly overlapping genes for liver transplantation and another 20 for kidney transplantation using leading edge analysis. Fourteen hub nodes （gene） for liver transplantation and 13 for kidney transplantation were identified by cytoscape software using interaction network analysis. Five core genes related to liver transplantation and 5 to kidney transplantation were obtained by integrating GSEA and biological network analysis. Conclusions Controlling the transcription and translation of the genes of the peripheral blood immune cells is the main immune regulation mechanism in liver transplantation recipients, but in the recipients of kidney transplantation, the protein interaction network plays a more prominent role. Energy metabolism and functional regulation of the immune cells are closely related. The core genes in peripheral blood immune cells related to liver or kidney transplantation may play key roles in regulating immune functions.

关 键 词：肝移植 肾移植 基因表达谱 基因集富集分析 生物网络 

分 类 号：R657.3[医药卫生—外科学] R699[医药卫生—临床医学]