中医骨折分期治疗对家兔骨折愈合过程中TGF-β与bFGF表达影响的实验研究  

Effects on TGF-β and bFGF expression of the tissues of periosteum in three period treatment of TCM for the fractures in rabbit

作　　者：毕锴[1] 温建民[1] 孙永生[1] 孙卫东 徐颖鹏[2] 胡海威[1] 桑志成[1] 高国庆[3] 董颖 

机构地区：[1]中国中医科学院望京医院,北京100102 [2]中国中医科学院广安门中医院 [3]怀柔区卫生局 [4]怀柔区社区康复中心

出　　处：《中国矫形外科杂志》2013年第4期381-386,共6页Orthopedic Journal of China

基　　金：国家自然基金资助项目(编号:30973762)

摘　　要：[目的]观察中医骨折三期治疗在家兔骨折模型愈合过程中,转化生长因子β(TGF-β)与碱性成纤维细胞生长因子(bFGF)表达的影响,探讨骨折中药分期论治的作用机制。[方法]选用174只成年健康雄性新西兰家兔,均建立桡骨远端骨折模型,利用计算机产生随机数字的方法,174只家兔随机取出6只作为正常对准组,其余分为四组,分别为中药三期治疗组、中药二期治疗组、中药一期治疗组、模型对照组,每组42只。每组又分为3、6、9、14、28、42、56 d 7个时间点,每个时间点6只,三期组分早、中、晚期分别予加减肢伤一方、二方与三方治疗,二期组早期予肢伤一方;后期予肢伤二方与三方的综合方治疗,一期组前三方综合方治疗,正常对准组和模型组给予等量蒸馏水。每组于不同时间点分别处死6只,取材,常规组织学检查,免疫组化法检测标本中的TGF-β与bFGF,应用计算机图像分析系统进行定量分析。[结果]各中药治疗组对于骨折端外骨膜、内骨膜和骨髓内组织TGF-β与bFGF表达平均阳性细胞平均光密度(MOD)值:二期组>三期组>一期组>模型组(P<0.05)。[结论]中药治疗能显著促进骨折愈合过程中TGF-β与bFGF的表达,二期治疗明显强于三期治疗、一期治疗,骨折分期辨证治疗的最佳分期为二期治疗。[ Objective ] To observe the effects of the different period treatment theory of bone fracture in TCM （ Traditional Chinese Medicine）on TGF-β and bFGF expression in the issues of outer periosteum, endosteum and bone marrow of rabbits, and to explore the rationality of phasing method in TCM in treating fracture, and which is the best method. [ Method ] 3 mm bone fracture line were made at lower one third part of both radius in 174 male healthy rabbits. Six rabbits were randomly taken into nomal group from these rabbits, and others were randomly divided into four groups, every group had 42 rabbits, including three period treatment group （ TTG）, two period treatment group （ TTG）, one period treatment group （ OTG）, model control group （MCG）. TTG were treated with three period treatment, two period treatment for WTG, and OTG were treated with one period treatment,rabbits in MCG were given no prescription or drug but distilled water as same dose as that of other groups. At day 3, 6,9,14,28,42 and 56, six rabbits from every group were randomly selected and were killed by aeroembolism. The left radiuses were taken out as the research object. Immunohistochemistry stain was performed to examinate the TGF-β and bFGF expression in the outer periosteum,endosteum and bone marrow. [ Result] The TGF-β and bFGF expression of all TCM treatment groups were enhanced in the outer periosteum, endosteum and bone marrow at different time points in fracture healing. The TGF-β and bFGF expression in the two tissues of TYG had the tendency of higher than that of the other groups at the most time points after operation. [ Conclusion] Treating fracture in stages has more predominant effect on the expression of TGF-β and bFGF than thatof treating fracture with single prescription or drug. The period treatment for fracture is very necessary, and the two period treatment is the best method.

关 键 词：骨折 转化生长因子Β 碱性成纤维细胞生长因子 免疫组化 兔 

分 类 号：R274.1[医药卫生—中医骨伤科学]