基于双光子激发荧光的单分子探测技术研究  

Experimental research of single-molecule detection by two-photon excited fluorescence

作　　者：朱峰[1] 马万云[1] 张亮[1] 文克玲[1] 陈瓞延[1] 

机构地区：[1]清华大学物理系,单原子分子测控教育部重点实验室,北京100084

出　　处：《原子与分子物理学报》2000年第1期59-62,共4页Journal of Atomic and Molecular Physics

基　　金：国家自然科学基金!编号 2 9875 0 12 ( 1999~ 2 0 0 1)

摘　　要：介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置,激发光聚焦和荧光收集采用共焦方式。选择香豆素C445水溶液作为研究对象,从样品流速、浓度、激光功率、信噪比和检测限等方面探讨了双光子激发荧光的特性。该谱仪目前己达到探测灵敏区内C445的平均分子数为1.We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence. Using aqueous C445 solution as the sample, the flow velocity, laser power, concentration linearity, and signal to noise ratio have been studied. Using the microscopy objective of 40×NA 0.65, the detection limit about 7.0×10 -12 mol/L was obtained, the average number of C445 in the sensitive volume (0.35pL) was about 1.5.

关 键 词：单分子探测 双光子激发荧光 C445 

分 类 号：O561.2[理学—原子与分子物理]