SD大鼠2型糖尿病模型的建立  

Establishment of type 2 diabetes rat model

作　　者：于纪棉[1] 张岳灿[1] 张玉琳[1] 张玲[1] 李高文[1] 王建峰[2] 

机构地区：[1]宁波卫生职业技术学院,315100 [2]宁波出入境检验检疫局

出　　处：《浙江医学》2012年第24期1958-1959,1963,共3页Zhejiang Medical Journal

基　　金：浙江省自然科学基金（LY12H07001）;宁波市自然科学基金（2011A610030）

摘　　要：目的利用高糖、高脂饮食联合注射链脲佐菌素（STZ），建立SD大鼠2型糖尿病动物模型，为抗糖尿病药物的筛选以及实验提供一个良好的动物模型。方法35只雄性SD大鼠随机分为实验组和对照组，实验组（25只）饲喂高糖、高脂饲料，对照组（10只）饲喂基础饲料。饲喂4周后，按30mg／kg剂量一次性腹腔注射STZ，对照组注射同等剂量的柠檬酸缓；中液。实验组继续饲喂高糖、高脂饲料，注射后每周采血1次，连续采3周，比较实验组与对照组空腹血糖、INS、血脂的变化。注射STZ后3周，剖杀所有实验大鼠，取胰腺，显微镜下观察胰岛的变化。结果实验组大鼠2型糖尿病特征显著，成功率高达88％。与对照组相比，实验组大鼠空腹血糖、INS、TC、TG、LDL以及HOMA-IR明显升高（P〈005或001）。结论高糖、高脂饮食加以一次性注射小剂量STZ可成功建立SD大鼠2型糖尿病模型。Objective To establish type 2 diabetic model in rats. Methods Thirty five male SD rats were randomly divid- ed into model group （n=25） and control group （n=10）. The rats in model group were fed with high-carbonhydrate-fat diet and rats in control group were fed with basic diet. After 4 weeks, the rats in model group received one-off intraperitoneal injection of streptozotocin at a dose of 30 mg/（kg .BW）; and the control group was injected with the same volume of citrate sodium buffer. Af- ter injection the model group was fed continually with high-earbonhydrate-fat feeds, and the blood samples were collected for measurement of fasting blood glucose, serum insulin and blood fat in the first, second and third week. All animals were sacrificed and pancreatic glands were collected for histopathological examination. Results At the end of experiments 88% rats in model group presented characteristic diabetic manifestations. The blood glucose levels （P〈0.01）, serum insulin levels （P〈0,01）, total cholesterol （P〈0.05）, triglyceride （P〈0.05）, low density lipoprotein （P〈0,05） and insulin resistance index （P 〈 0.05） in the model group were increased significantly compared with the control group. Conclusion Type 2 diabetic model has been established successfully by feeding with high-carbonhydrate-fat diet and one-off intraperitoneal injection with streptozotocin in SD rats.

关 键 词：SD大鼠 2型糖尿病 动物模型 

分 类 号：R286.715[医药卫生—中药学]