郁金提取物对小鼠的镇痛作用及其机制的研究  

Analgesic effect of Radix Curcuma extracts and its mechanism

作　　者：龚敏操[1] 许俊杰[2] 陈眉[1] 

机构地区：[1]浙江省中医院神经内科,杭州310006 [2]嘉兴市第一医院神经内科

出　　处：《浙江医学》2012年第24期1968-1970,共3页Zhejiang Medical Journal

基　　金：浙江省中医药科技基金资助项目（2009CA012）

摘　　要：【摘要】目的观察郁金提取物对疼痛小鼠的干预作用及其机制的探讨。方法采用甲醛实验，观察各组小鼠I、Ⅱ相累计舔足时间；行为学观察后取血处理，用放射免疫法测定外周血清TNF-α的含量并进行比较。结果单侧足底皮下注射甲醛能使小鼠出现明显的两相伤害性行为反应；郁金提取物能抑制甲醛引起的第Ⅱ时相疼痛行为反应，3个剂量组的镇痛作用和模型组相比，差异均具有统计学意义（均P〈0．01）；其中郁金提取物300mg／kg剂量组镇痛作用与阿司匹林组相比，差异具有统计学意义（P〈0．05）。各组小鼠外周血清中TNF-α含量与空白组相比，均有明显升高，差异均有统计学意义（均P〈0．01）；与造模组相比，郁金提取物各组均能明显降低小鼠血清TNF-α含量（均P〈001）；其中郁金提取物300mg／kg剂量组降低至正常水平，与空白对照组相比，无统计学差异（P〉0．05），甚至明显低于阿司匹林组TNF-α含量（P〈005）。结论郁金提取物具有明显的镇痛作用，其作用部位可能在外周，镇痛机制可能跟降低炎症细胞因子的表达，参与抑制炎症反应有关。Objective To investigate the effects of Radix Curcuma extracts on inflammatory pain in mice and its mecha- nism. Methods The formalin test was adopted to observe the accumulative licking foot times of each phases in mice. The serum levels of tumor necrosis factor-a（TNF- α） were measured by radioimmunoassay. Results Subcutaneous injection of formalin into mouse foot elicited acute two-phase noxious reaction. The second phase of pain reaction induced by formalin was decreased af- ter administration of Radix Curcuma extracts （P〈0.01） in a dose-dependent manner. The Radix Curcuma extracts at 300mg/kg showed a better analgesic effect than that of aspirin （P〈0.05）. The TNF- α level was significant increased in model group com- pared to control group （P〈0.01）. Radix Curcuma extracts decreased serum TNF-α levels compared to the model group in a dose-dependent manner （P〈0.01）; TNF- α level in 300mg/kg-dose group was even lower than the baseline level. Conclusion Radix Curcuma extracts have analgesic effects in mice, which may be associated with inhibition of peripheral inflammation.

关 键 词：郁金 β-榄香烯镇痛作用 甲醛实验肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]