肺炎支原体的分离鉴定与药敏性研究  

Isolation,Identification and Drug Sensitivity of Mycoplasma Pneumoniae

作　　者：刘展[1] 袁春丽[1] 李青凤[2] 汪水城 王本旭[2] 

机构地区：[1]北京市怀柔区妇幼保健院,北京101400 [2]北京军区疾病预防控制中心,北京100042

出　　处：《中国临床医学》2012年第6期622-623,共2页Chinese Journal of Clinical Medicine

基　　金：北京市首都医学发展科研基金(编号:20093267)

摘　　要：目的:探讨肺炎支原体的临床分离株对临床常用药的敏感性。方法:将临床采集的咽试子标本经过选择性培养基筛选培养,同时进行聚合酶链反应(polymerase chain reaction,PCR)检测,确定分离到的支原体为肺炎支原体。将肺炎支原体与不同浓度的临床常用抗生素混合,观察液体培养基颜色的变化,判断肺炎支原体对药物的敏感性。结果:成功分离到1株肺炎支原体;阿奇霉素、红霉素和头孢呋辛钠浓度分别稀释至31μg/mL、125μg/mL、500μg/mL时,肺炎支原体抑制;而头孢曲松钠、美洛西林、氨苄青霉素等抗生素对支原体无抑制作用,培养基颜色均由红色变为橙黄色。结论:肺炎支原体临床分离株对阿奇霉素高度敏感,对红霉素和头孢呋辛钠中度敏感。Objective：To investigate the drug sensitivity of mycoplasma pneumoniae isolated from patients. Methods： Samples of throat swab were cultivated in selective culture medium, and then they were detected by PCR. Mycoplasma pneumoniae were mixed with different quantity of antibiotics. If one drug could kill the microorganism, the color of the media would not change. Results： One clinical mycoplasma pneumoniae type was isolated successfully. When the concentration of azithromycin, erythro- mycin and cefuroxime sodium were diluted to 31 μg/mI., 125 μg/mL, 500/μg/mL, respectively, mycoplasma pneumoniae were supressed. Ceftriaxone sodium, mezlocillin and ampicillin had no effect on the growth of mycoplasma pneumoniae. Conclusions： The growth of mycoplasma pneumoniae isolated from throat swab samples is inhibited easily by azithromycin. Erythromycin and cefuroxime sodium also had inhibitory effect on mycoplasma pneumoniae.

关 键 词：肺炎支原体 分离鉴定 药敏性 

分 类 号：R725.6[医药卫生—儿科]