Enzyme Activity in Water-Stable Soil Aggregates as Affected by Long-Term Application of Organic Manure and Chemical Fertiliser  

作　　者：LIU Yi-Ren LI Xiang SHEN Qi-Rong XU Yang-Chun 

机构地区：[1]Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization, College of Resources and Environmental Sciences,Nanjing Agricultural University [2]Institute of Soil Fertilizer and Resource Environment, Jiangxi Academy of Agricultural Sciences

出　　处：《Pedosphere》2013年第1期111-119,共9页土壤圈（英文版）

基　　金：Supported by the National Basic Research Program (973 program) of China (No. 2007CB109304);the Special Fund for Agroscientific Research in the Public Interest of China (No. 200803031)

摘　　要：The activities of invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase in different fractions of waterstable aggregates (WSA) were examined in long-term (26 years) fertilised soils. The long-term application of organic manure (OM) with chemical fertiliser (CF) significantly increased macroaggregate and decreased microaggregate percentages, enhanced the mean weight diameter, and significantly increased soil total carbon (TC) and total nitrogen (TN) contents of WSA in different size fractions. Combined fertilisation with OM and CF also increased invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase activities of WSA in different size fractions. Enzyme activities were higher in macroaggregates than in microaggregates. The distribution of enzyme activities generally followed the distribution of TC and TN in WSA. The geometric mean of the enzyme activities in different WSA of OM-treated soils was significantly higher than that in soils treated with 100% CF or no fertiliser. The results indicated that the long-term combined application of OM with CF increased the aggregate stability and enzyme activity of different WSA sizes, and consequently, improved soil physical structure and increased soil microbial activity.The activities of invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase in different fractions of water- stable aggregates （WSA） were examined in long-term （26 years） fertilised soils. The long-term application of organic manure （OM） with chemical fertiliser （CF） significantly increased macroaggregate and decreased microaggregate percentages, enhanced the mean weight diameter, and significantly increased soil total carbon （TC） and total nitrogen （TN） contents of WSA in different size fractions. Combined fertilisation with OM and CF also increased invertase, protease, urease, acid phosphomonoesterase, dehydrogenase, and catalase activities of WSA in different size fractions. Enzyme activities were higher in macroaggregates than in microaggregates. The distribution of enzyme activities generally followed the distribution of TC and TN in WSA. The geometric mean of the enzyme activities in different WSA of OM-treated soils was significantly higher than that in soils treated with 100% CF or no fertiliser. The results indicated that the long-term combined application of OM with CF increased the aggregate stability and enzyme activity of different WSA sizes, and consequently, improved soil physical structure and increased soil microbial activity.

关 键 词：DISTRIBUTION mean weight diameter stability total carbon total nitrogen 

分 类 号：S158[农业科学—土壤学]