人卵巢组织中HBVDNA和HBVcccDNA的表达与HBV宫内感染的相关性研究  

Relationship between the expression of HBV DNA, HBV cccDNA in human ovary tissues and the HBV intrauterine infection

作　　者：余敏敏[1] 顾小军[1] 夏茵[1] 王根菊[1] 阚乃颖[1] 吴凯华[1] 

机构地区：[1]东南大学附属第二医院,南京210003

出　　处：《中华流行病学杂志》2013年第2期178-182,共5页Chinese Journal of Epidemiology

基　　金：基金项目：江苏省自然科学基金（BK2008070）

摘　　要：目的研究人卵巢组织中乙型肝炎病毒（HBV）DNA、HBV共价闭合脱氧核糖核酸（cccDNA）表达与HBV宫内感染的相关性。方法采用荧光定量聚合酶链反应方法（FQ—PCR）检测33例HBVDNA阳性孕妇卵巢组织中的HBVDNA和HBVcccDNA。采用化学发光法检测相应33例婴儿出生当日和1月龄外周血血清乙型肝炎病毒标志物（HBVM），FQ—PCR法检测婴儿血清HBVDNA含量。结果33例卵巢组织中HBVDNA和HBVcccDNA总阳性率为51．52％（17／33）。婴儿宫内感染率为12．12％（4／33，4例均为肝功能正常的孕妇）。婴儿母亲卵巢组织中HBVDNA和HBVcccDNA均阳性时，宫内感染率比HBVDNA、HBVcccDNA均阴性时显著升高（P〈0．05）。宫内感染婴儿较非宫内感染婴儿母亲卵巢组织中HBVcccDNA的表达水平和阳性率明显升高（P〈0．01和P〈0．05）。结论HBV可感染人卵巢组织并在其中复制，且有可能通过卵细胞垂直传播至子代。Objective To investigate the relationship between hepatitis B virus （HBV） deoxyribonucleic acid （DNA） and HBV eovalently closed circular DNA （cccDNA） in the ovary and HBV intrauterine infection. Methods HBV DNA and HBV cccDNA were assayed in the ovaries of 33 pregnant women who were positive for HBV DNA, tested by Fluorescent quantitative polymerase chain reaction （FQ-PCR）. The level of HBV mark （HBVM） and the content of HBV DNA in peripheral blood of infants were measured by chemoluminescence and FQ-PCR methods respectively. Results The overall positive rate for both HBV DNA and HBV cccDNA in ovarian samples was 51.52% （17/33）. The rate on intrauterine infection among infants was 12.12% （4/33） and all the 4 infected infants were delivered from mothers with normal hepatic function. When HBV DNA and HBV cccDNA were both positive, the rate of intrauterine infection in infants was significantly higher than those who were with both negative results （P〈0.05）. Levels of HBV cccDNA and the rate of positive samples were significantly higher in mothers with infants who appeared to have had intrauterine infection than those did not （P〈0.01 and 〈0.05, respectively）. Conclusion HBV infection could be discovered in the human ovary and might be transmitted to the filial generation via ovum.

关 键 词：乙型肝炎病毒 宫内感染 卵巢 

分 类 号：R512.62[医药卫生—内科学]