槲皮素对SiO_2介导人胚肺成纤维细胞增殖及TGF-β1表达的影响  

Effect of quercetin on the expression of TGF-β1 in human embryonic lung fibroblasts activated by the silicotic alveolar macrophages

作　　者：彭海兵[1] 刘燕[2] 王建行[1] 赵霞[1] 王献华[2] 

机构地区：[1]河北联合大学冀唐学院,唐山063000 [2]河北联合大学基础医学院

出　　处：《卫生研究》2013年第1期99-102,共4页Journal of Hygiene Research

基　　金：河北省自然科学基金资助项目(No.C2008001007);河北省科学技术研究与发展计划项目(No.09276199D)

摘　　要：目的探讨槲皮素抗硅肺纤维化的作用机制。方法以支气管肺泡灌洗法收集硅肺患者AM,在体外用含有SiO2(50μg/ml)的DMEM培养基和不含SiO2的DMEM培养基培养18h,然后收集培养的AM上清液,加入低、中、高浓度槲皮素(10、20和40μmol/L)作为刺激上清。用组织贴块法获取培养HELF,与刺激上清液共同孵育后,MTT法检测HELF增殖情况,免疫细胞化学法检测HELF中TGF-β1的表达。结果 (1)各槲皮素组能抑制HELF增殖,并且浓度愈大抑制作用愈强,三组与SiO2+AM组(0.620±0.096)相比、中浓度组(0.406±0.070)和高浓度组(0.334±0.050)与AM组(0.490±0.064)相比差异有显著性(P<0.01)。(2)三种浓度槲皮素均能降低HELF中TGF-β1的表达,与SiO2+AM组(0.542±0.095)相比差异有显著性(P<0.05),与AM组(0.394±0.109)和空白对照组(0.314±0.066)比较差异无显著性。结论槲皮素对硅肺纤维化有一定的防治作用。Objective To explore the effect s of quercetin （Que） intraperitoneal pulmonary fibrosis. Methods The silicotic alveolar macrophages were collected by bronehoalveolar lavage andincubated in vitro in the DMEM medium containing SiO2 （50μg/ml） and DMEM medium without SiO2 for 18h. Then the AM supernatant incubated for 18h was collected and with quercetins （10, 20 and 40μmol/L） as stimulus supernatant. HELFs were isolated by organize paste block method, and incubated with stimulus supernatants. The proliferation in the HELF was detected with MTT method. The expressions of TGF-β1 in the HELF are detected with the immunoeytochemistry method. Results （1）Three groups of Que could inhibit the proliferation of HELF, and more concentration, inhibition is stronger. Three groups of Que compared with SiO2 ＋ AM group （0.620±0.096）, Que20 group（0.406 ±0.070）and Que40 group （0.334 ±0.050）compared with AM group （0. 490±0. 064）, differences were significant （P 〈 0.01 ）. （2） Three groups of Que could reduce the expression of TGF-β 1 in HELF, and compared with SiO2 ＋ AM group （0. 542 ± 0. 095） differences were significant（P 〈 0.05 ）. Three groups of Que compared with AM group （0. 394 ±0. 109） and blank control group （0. 314 ± 0. 066） differences were not significant. Conclusions Quercetin could prevent and treat pulmonary fibrosis stimulated by silicon dioxide.

关 键 词：槲皮素 SIO2 TGF-Β1 肺纤维化 肺泡巨噬细胞 人胚肺成纤维 细胞 

分 类 号：R135.2[医药卫生—劳动卫生] R282.711[医药卫生—公共卫生与预防医学]