明日叶查尔酮对2型糖尿病大鼠肝脏和骨骼肌葡萄糖转运体表达的影响  

Effects of angelica keiskei chalcone on expression of glucose transporter proteins in liver and skeletal muscle cells of type 2 diabetic rats

作　　者：赵阳[1] 刘贝[1] 李蕾[2] 钟进义[1] 

机构地区：[1]青岛大学医学院公共卫生系,青岛266021 [2]厦门大学公共卫生学院

出　　处：《卫生研究》2013年第1期107-109,113,共4页Journal of Hygiene Research

基　　金：山东省卫生厅青年基金(No.QNW001)

摘　　要：目的研究明日叶查尔酮对2型糖尿病大鼠肝脏葡萄糖转运体2(Glut2)和骨骼肌葡萄糖转运体4(Glut4)蛋白表达的影响。方法制造2型糖尿病大鼠模型,随机分成高、中、低剂量组和糖尿病对照组,分别每日灌胃给予30、10、5和0mg/kg BW明日叶查尔酮。各组以高脂饲料喂养4周后,用葡萄糖氧化酶法检测空腹血糖;放射免疫法检测血清胰岛素含量;免疫组化法检测葡萄糖转运体蛋白表达水平。结果经图像分析,高剂量组肝脏和骨骼肌葡萄糖转运体蛋白表达平均光密度值分别为(0.036±0.0030)和(0.063±0.0139),均较糖尿病对照组显著升高(P<0.05)。高剂量组空腹血糖和胰岛素水平分别为(12.3±1.64)mmol/L和(25.65±3.34)μIU/ml,均较糖尿病对照组显著性降低(P<0.05)。结论明日叶查尔酮可上调2型糖尿病大鼠肝脏葡萄糖转运体2和骨骼肌葡萄糖转运体4蛋白表达水平,降低空腹血糖和胰岛素水平,改善糖尿病胰岛素抵抗状况。Objective To investigate the effect of angelica keiskei chalcone（AC） on the expression of glucose transporter2 （Glut2） in liver and glucose transporter 4 （Glut4） in skeletal muscle cells of rats with type2 diabetes. Methods Made type2 diabetic rat models and divided into high,middle,low dose groups and diabetic control group randomly which were given chalcone 30,10,5 and Omg/kg BW respectively. Four groups were fed with the diet containing high fat. After four weeks, fasting blood glucose was determined by glucose oxidase method. Insulin was assessed by radio-immunity method. The expression levels of Glut2 in liver and Glut4 in skeletal muscle cells were analyzed by immunohistochemistry method. Results After analyzed by Image-Pro Plus on immunohistochemistry pictures, the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells in high group were （0. 036 ± 0. 0030） and （0. 063± 0. 0139 ）,which were significantly higher than the model control group （ P 〈 0.05 ）. The levels of fasting blood glucose and insulin in high dose group were （ 12.3 ±1.64） mmol/L and （25.65± 3.34） μIU/ml, significantly lower than those of the model control group （P 〈 0.05）. Conclusion Angelica keiskei chalcone may increase the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells, decrease fasting blood glucose and insulin of type 2 diabetic rats and improve their insulin resistance condition.

关 键 词：明日叶查尔酮 2型糖尿病 葡萄糖转运体 

分 类 号：R587.1[医药卫生—内分泌] R282.71[医药卫生—内科学]