同源性金黄色葡萄球菌生物被膜形成能力比较  

Biofilm formation ability of homologous Staphylococcus aureus strains:a comparative study

作　　者：孔晋亮[1] 张东伟[2] 陈一强[1] 闫萍[1] 蔡双启[1] 简丽娟[1] 

机构地区：[1]广西医科大学第一附属医院呼吸内科,广西南宁530021 [2]柳州市人民医院呼吸内科,广西柳州545001

出　　处：《中华医院感染学杂志》2013年第3期481-483,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81060002);广西研究生教育创新计划(2009105981002M209);广西壮族自治区卫生厅课题(Z2010334);广西壮族自治区中医药管理局基金课题(gzzc1138);广西壮族自治区教育厅(桂教201106LX092)

摘　　要：目的比较临床分离同源性金黄色葡萄球菌(SAU)的黏附及生物被膜(BF)形成能力差异。方法刚果红平板法定性能形成BF的菌株;建立SAU的BF体外静置模型,分别于建模第1、3、7天,采用结晶紫染色方法,比较临床分离同源性SAU的黏附及BF形成能力差异。结果临床分离10株同源性SAU中,刚果红平板法定性可全部形成BF;SAU 17546的黏附及形成早期BF能力最强(P<0.001),而SAU 17422最弱(P<0.001);形成成熟BF能力,仍以SAU 17546最强(P<0.001),但SAU 17642与17546比较,两者极近似(P=0.495);SAU17422形成BF能力最弱,但是与17431、18541-2、18558、18565、18719等菌株差异无统计学意义。结论刚果红平板法可定性SAU形成BF菌株,同源性SAU黏附及形成BF的能力存在差异。OBJECTIVE To compare the adhesion and the abilities of biofilm formation of the homologous clinical isolates of Staphylococcus aureus.METHODS Congo red plate method was used to detect strains The in vitro biofilm model of S.aureus was established,and the crystal violet staining biofilm semi-quantitative method was employed to compare the adhesion and biofilm formation abilities of the homologous S.aureus.RESULTS Totally 10 strains of homologous S.aureus were biofilm formation-positive,which were isolated from the clinic.The ability of adhesion and early biofilm formation of S.aureus 17546 was the strongest（P0.001）,but S.aureus 17422′s was the weakest（P0.001）.As for the formation ability of the mature biofilm,S.aureus 17546 was the most powerful（P0.001）,S.aureus 17642 was extremely similar to strain 17546（P=0.495）.the biofilm formation ability of S.aureus 17422 was the weakest,but as compared with S.aureus 17431,S.aureus 18541-2,S.aureus 18558,S.aureus 18565 and S.aureus 18719,the difference was not statistically significant.CONCLUSION Congo red plate method can detect the S.aureus strains of BF formation.All the homologous S.aureus strains vary in the adhesion and biofilm formation ability.

关 键 词：同源性 金黄色葡萄球菌 生物被膜 

分 类 号：R378.1[医药卫生—病原生物学]