血培养3种采血方式结果分析  

Three blood sampling approaches for blood culture

作　　者：陆文香[1] 吴培南 徐卫东[1] 

机构地区：[1]苏州市立医院本部检验科,江苏苏州215002

出　　处：《中华医院感染学杂志》2013年第3期712-714,共3页Chinese Journal of Nosocomiology

摘　　要：目的分析单侧单瓶、单侧双瓶、双侧双瓶采血方式的血培养结果。方法回顾性分析2011年1-12月采用单侧单瓶(需氧瓶)、成人双侧双瓶(两套需氧+厌氧)、婴幼儿患者单侧双瓶(需氧+厌氧)采血结果,进行血培养阳性率的差异及污染菌的鉴别。结果成人患者血培养阳性率单侧单瓶8.8%、双侧双瓶16.0%,两者之间差异有统计学意义(P<0.05);婴幼儿患者血培养阳性率单侧单瓶4.5%、单侧双瓶5.0%,两者之间差异无统计学意义;单侧单瓶共检出病原菌45株,20株为可疑污染菌,占44.4%;双侧双瓶检出病原菌68株,24株为可疑污染菌,其中12株从多份培养瓶中检出,确定为病原菌,其余12株仅从单份培养瓶中检出,判断为污染菌;单侧双瓶检出病原菌62株,36株为可疑污染菌,其中20株仅从1瓶中检出,判断为污染菌。结论成人患者血培养双侧双瓶与单侧单瓶采集相比,有明显优势;婴幼儿血培养单侧双瓶与单侧单瓶采集相比,对可疑污染菌的鉴别有一定优势,但阳性率提高并不明显,鉴于婴幼儿厌氧菌血流感染比较罕见,建议改单侧双瓶(需氧+厌氧)为双侧单瓶(需氧+需氧)。OBJECTIVE To analyze the blood culture approaches with unilateral single bottles, bottles of unilateral double, and bilateral double bottles. METHODS The blood samplings were performed by respectively adopting the unilateral single bottle （aerobic bottle）, bilateral double bottle for the adult patients （two sets of aerobic and anaerobic）, and the unilateral double bottle for infants （aerobic and anaerobic）, the difference between the positive rates of blood culture was observed, and the contaminated bacteria were identified. RESULTS The positive rate of unilateral single bottle for the adult patients was 8.8~//oo, bilateral double bottle 16.0%, the difference was statisti- cally significant （P^0.05）. The positive rate of the unilateral single bottle for the infants was 4.5~, unilateral double bottle 5.0%, the difference was not statistically significant （P~〉0.05）. A total of 45 strains of bacteria were detected by means of the unilateral single bottle. Totally 68 strains were isolated with the bilateral double bottle, 24 strains were suspicious contaminated bacteria, of which 12 strains were detected from multiple bottle, being judged as pathogenic bacteria, and the remaining 12 strains were detected from the single culture bottle, being judged as contaminated bacteria. Totally 62 strains were isolated from unilateral double bottle, 36 strains were suspicious contaminated bacteria, 20 strains were detected with the single culture bottle, being judged as the contaminated bacteria. CONCLUSION The blood culture with the bilateral double bottle collection for the adult patients has a distinct advantage, as compared with unilateral single bottle collection, in the increasing of positive rate or the identification of the contaminating bacteria. The blood culture with unilateral double bottle collection for the infants has the advantage, in a certain extent, in the identification of the contaminating bacteria as com- pared with unilateral single bottle collection, without the positive rate increasi

关 键 词：血培养 采血方式 阳性率 污染菌 

分 类 号：R446[医药卫生—诊断学]