Identification of Novel and Differentially Expressed MicroRNAs in the Ovaries of Laying and Non-Laying Ducks  

作　　者：YU De-bing JIANG Bao-chun GONG Jing DONG Fu-lu LU Ying-lin YUE Hui-jie WANG Zheng-chao DU Wen-xing GUO An-yuan 

机构地区：[1]Department of Animal Science and Technology, College of Animal Science and Technology, Nanjing Agricultural University [2]Bioinformatics & Molecular Imaging Key Laboratory, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology [3]Provincial Key Laboratory for Developmental Biology and Neurobiology, College of Life Science, Fujian Normal University

出　　处：《Journal of Integrative Agriculture》2013年第1期136-146,共11页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31101705);the Natural Science Foundation of Jiangsu Province, China (BK2010454);the Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province, China (cx(09)116 and cx(11)1028)

摘　　要：MicroRNAs （miRNAs）, which post-transcriptionally regulate gene expression by binding to the 3′untranslated region of mRNAs to either inhibit or enhance translation, are involved in diverse biological processes. The use of high-throughput Solexa sequencing plays important roles in the discovery of miRNAs. In this study, we used high-throughput Solexa sequencing to identify novel duck miRNAs and compare the miRNA expression profiles in laying and non-laying duck ovaries. Using a bioinformatic analysis, we discovered 86 potential duck miRNAs similar to known chicken miRNAs and 43 unique sequences that matched known miRNAs of other species. We also found that miRNA variations and isoforms were widespread in our two RNA libraries, with most of the variations occurring in the 3′region of the miRNAs. Furthermore, we detected 55 miRNAs that exhibited significant expression differences between laying and non-laying ducks. Gene ontology （GO） and kyoto encyclopedia of genes and genomes （KEGG） enrichment analyses of the potential targets of the differentially expressed miRNAs indicated these miRNAs may play key roles in the egg laying process. Finally, we confirmed the differential expression of 5 miRNAs in the laying and non-laying samples by qRT-PCR. Cumulatively, our work provides the first look at the miRNA expression profile of the duck ovary and provides novel insight into the roles of miRNAs in egg laying and reproduction.MicroRNAs （miRNAs）, which post-transcriptionally regulate gene expression by binding to the 3′untranslated region of mRNAs to either inhibit or enhance translation, are involved in diverse biological processes. The use of high-throughput Solexa sequencing plays important roles in the discovery of miRNAs. In this study, we used high-throughput Solexa sequencing to identify novel duck miRNAs and compare the miRNA expression profiles in laying and non-laying duck ovaries. Using a bioinformatic analysis, we discovered 86 potential duck miRNAs similar to known chicken miRNAs and 43 unique sequences that matched known miRNAs of other species. We also found that miRNA variations and isoforms were widespread in our two RNA libraries, with most of the variations occurring in the 3′region of the miRNAs. Furthermore, we detected 55 miRNAs that exhibited significant expression differences between laying and non-laying ducks. Gene ontology （GO） and kyoto encyclopedia of genes and genomes （KEGG） enrichment analyses of the potential targets of the differentially expressed miRNAs indicated these miRNAs may play key roles in the egg laying process. Finally, we confirmed the differential expression of 5 miRNAs in the laying and non-laying samples by qRT-PCR. Cumulatively, our work provides the first look at the miRNA expression profile of the duck ovary and provides novel insight into the roles of miRNAs in egg laying and reproduction.

关 键 词：microRNA Solexa sequencing DUCK OVARY 

分 类 号：S834[农业科学—畜牧学]