红车轴草总异黄酮成分DNA结合剂的超滤质谱筛选  

Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MS^n

作　　者：马蕾[1,2] 王兆伏[1] 陈丽娜[1,2] 宋凤瑞[1] 刘志强[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所,长春质谱中心,长春130022 [2]中国科学院大学,北京100049

出　　处：《高等学校化学学报》2013年第2期331-335,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21073178,21175128)资助

摘　　要：采用离心超滤和液相色谱-质谱联用的方法,从红车轴草异黄酮提取物中筛选DNA结合剂.结果表明,红车轴草中10种异黄酮成分与DNA具有不同的结合能力.采用液相色谱-串联质谱联用技术对其结构进行了鉴定,DNA结合能力较强的5种化合物分别是德鸢尾素-4'-O-β-D-葡萄糖苷、德鸢尾素、黄豆黄葡萄糖苷、红车轴草素和鹰嘴豆牙素A.为从中药提取物等复杂体系筛选并鉴定DNA结合剂建立了快速的超滤质谱平台.Centrifugal ultrafiltration and LC-MS methods were combined to screen DNA-binding agents from traditional Chinese medicine red clover isoflavone extracts.The results show that 10 red clover isoflavones have different binding degrees with DNA.The sample was analyzed by LC-MSn for identifying structures.Among these flavones studied,the strongest five potential DNA-binding agents are irilone-4′-O-β-D-glucoside,glycitin,pratensein,irilone and biochanin A,respectively.A rapid centrifugal ultrafiltration-LC-MS platform for screening and identification of DNA binders from the herbal extract and other complex system was established.

关 键 词：红车轴草 DNA 离心超滤 液质联用 

分 类 号：O657.63[理学—分析化学]