雷公藤多苷主要有效成分的含量研究  

Determination of main effective components in tripterygium glycosides

作　　者：何昱[1] 石森林[1] 张茹萍[1] 钱家健[1] 范永升[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《药物分析杂志》2013年第2期197-200,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：浙江省重点科技创新团队项目(2011R09042-06);浙江省教育厅科研项目(Y201120445)

摘　　要：目的:测定雷公藤多苷中雷公藤甲素、雷公藤内酯酮、雷公藤晋碱、雷公藤次碱、雷公藤红素、雷公藤内酯甲6个有效成分的含量,为雷公藤多苷的质量评判以及组分与药效、毒性之间的关系探讨奠定基础。方法:采用高效液相色谱法同时测定雷公藤多苷中6个有效成分的含量,色谱柱为Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸-0.2%三乙胺为流动相梯度洗脱,流速0.75 mL.min-1,柱温35℃,检测波长225 nm(0～65 min)、210 nm(65～80 min)。结果:在所选择的分析条件下,较好地定量了样品中的各组分。6个有效成分雷公藤甲素、雷公藤内酯酮、雷公藤晋碱、雷公藤次碱、雷公藤红素、雷公藤内酯甲的线性范围分别为0.005～0.10 mg.mL-1(r=0.9998)、0.01～0.06 mg.mL-1(r=0.9995)、0.10～0.20 mg.mL-1(r=0.9993)、0.10～0.20 mg.mL-1(r=0.9997)、0.0025～0.20 mg.mL-1(r=0.9995)、0.015～0.07mg.mL-1(r=0.9997);平均加样回收率为98.74%(RSD=1.5%)、96.85%(RSD=1.3%)、98.66%(RSD=2.4%)、95.61%(RSD=2.6%)、99.26%(RSD=1.9%)、99.25%(RSD=2.7%)。结论:所建立的方法可用于雷公藤多苷有效成分的定量分析;不同厂家生产的雷公藤多苷化学组成存在着较明显的差异。Objective： To determine six effective components （ triptolide, triptonide, wilforgine, wilforine, tripterine, wilforlide A） in tripterygium glycosides, so as to provide reference for the quality evaluation and investigation on the relationship among chemical composition, effect and toxicity. Methods：The separation was achieved using an Agi- lent ZORBAX SB -C18 column（4. 6 mm ×250 mm,5 /zm） with an acetonitrile -0. 1% phosphoric acid -0. 2% tri- ethylamine gradient elution system. The column temperature was maintained at 35℃ and the flow rate was 0. 75 mL · min^-1. The elution was detected at 225 nm from 0 min to 65 min,and then at 210 nm from 65 min to 80 min. Re- stilts ： Under the selected analytical condition, the components in the samples were quantified precisely. The standard cur ves for triptolide, triptonide, wilforgine, wilforine, tripterine and wilforlide A were linear over the ranges of 0. 005 - 0. 10 nag · mL^ - 1 （ r = 0. 9998 ）, 0. 01 - 0. 06 mg · mL ^- 1 （ r = 0. 9995 ）, 0. 10 - 0. 20 mg · naL ^- 1 （ r = 0. 9993 ）, 0.10-0.20 nag· mL^-1（r=0.9997）,0.0025-0.20 mg · naL^-1（r =0.9995）,0.015 -0.07mg· mL^-1（r = 0. 9997） ,and the average recoveries were 98.74% （ RSD = 1.5% ） ,96.85% （ RSD = 1.3% ） ,98.66% （ RSD = 2. 4% ） ,95.61% （ RSD = 2.6% ）, 99.26% （ RSD = 1.9% ）, 99.25% （ RSD = 2. 7% ）, respectively. Conclusion： The established method is suitable for the determination of effective ingredients in tripterygium glycosides ; the differ- ences of contents are significant among samples from different manufacturers.

关 键 词：雷公藤多苷 雷公藤甲素 雷公藤内酯酮 雷公藤晋碱 雷公藤次碱 雷公藤红素 雷公藤内酯甲 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]