HPLC法测定枳壳中6个二氢黄酮类成分的含量  

Quantification of six flavonones in Aurantii Fructus by HPLC

作　　者：林宗涛[1] 陈世忠[1] 王弘[1] 

机构地区：[1]北京大学药学院,北京100191

出　　处：《药物分析杂志》2013年第2期201-205,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技重大专项-中药质量标准研究和信息化体系建设平台项目(2009ZX09308)

摘　　要：目的:建立HPLC法测定枳壳药材中6个黄酮类成分圣草次苷、新圣草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷的含量。方法:采用Inertsil ODS-4(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸(18∶82),流速1.0 mL.min-1,检测波长283 nm,柱温30℃。结果:圣草次苷、新圣草苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷6个二氢黄酮类化合物的质量分别在0.02～0.16、0.60～0.54、0.02～0.18、0.41～3.70、0.16～1.40、0.22～1.99μg范围内与峰面积呈良好的线性关系,r分别为0.9995、0.9999、0.9999、0.9999、0.9999和0.9999;平均加样回收率(n=6)分别为100.8%、102.5%、97.8%、99.6%、98.0%、102.7%。结论:含量测定方法可作为中药枳壳的质量控制的有效方法。Objective：To establish an HPLC method for simultaneous determination of eriocitrin, neoeriocitrin, na- rirutin, naringin, hesperidin, neohesperidin content in Aurantii Fructus. Methods： HPLC separation was carried out with an Inertsil ODS-4 column（4.6 mm× 150 ram,5 txm）using acetonitrile-0.1% phosphoric acid（ 18： 82）as mobile phase at a flow rate of 1.0 mL · rain^- 1. The detection wavelength was 283 nm and the column temperature was maintained at 30 ℃. Results： Good linear relationships were observed between the masses and the peak areas of eriocitrin, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin in the ranges of 0.02 - 0.16 （ r = 0. 9995 ）, 0.60 -0.54（r =0.9999） ,0.02 -0.18（r =0.9999） ,0.41 -3.70（r =0.9999） ,0.16 - 1.40（r =0.9999） and 0.22 - 1.99 （ r = 0. 9999 ） μg, respectively ; The average recovery rates （ n = 6 ） were 100.8%, 102.5%, 97.8%, 99.6 %, 98.0% and 102.7 %, respectively. Conclusion：The established quantification method is applicable for the quality control of Aurantii Fructus.

关 键 词：枳壳药材 二氢黄酮 圣草次苷 新圣草苷 芸香柚皮苷 柚皮苷 橙皮苷 新橙皮苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]