HPLC-DAD-ELSD法同时测定黄芪中5个成分的含量  

Simultaneous determination of five compounds in Radix Astragali by high-performance liquid chromatography coupled with diode arry and evaporative light scattering detectors

作　　者：梁瑾[1] 刘小花[1] 任远 封士兰[1] 

机构地区：[1]兰州大学药学院,兰州730000 [2]甘肃省中药药理与毒理学重点实验室,兰州730000

出　　处：《药物分析杂志》2013年第2期210-213,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：中央高校基本科研业务费专项资金(No.Lzujbky-2011-137);甘肃省科技重大专项项目(No.1002FKDA048);甘肃省中药药理与毒理学重点实验室开放基金项目(No.ZDSYS-KJ-2012-007)

摘　　要：目的:建立HPLC-DAD-ELSD法同时测定黄芪药材中黄酮类和皂苷类成分的方法,比较15批不同产地的黄芪药材中各有效成分的含量。方法:采用HPLC-DAD-ELSD联用法,乙腈和水不同比例梯度洗脱,流速1 mL.min-1,检测波长254nm;蒸发光散射检测器(ELSD)漂移管温度112.8℃,载气流速3.2 L.min-1,同时测定黄芪药材中毛蕊异黄酮、芒柄花素、黄芪甲苷(黄芪皂苷Ⅳ)、黄芪皂苷Ⅱ和黄芪皂苷Ⅲ的含量。结果:通过1次进样,同时测定了2个黄酮类和3个皂苷类等成分的含量,且线性关系良好(r=0.9992～0.9999)。平均回收率为99.1%～100.9%。结论:该方法简便,重复性好,可用于黄芪药材中黄酮类和皂苷类成分的同时测定,也可用于黄芪药材的质量控制。Objective：To establish a quantitative method of two major active isoflavonids and three main saponins in Radix Astragali by high -performance liquid chromatography coupled with diode arty and evaporative light scat- tering detectors（ HPLC -DAD- ELSD） , and compare the contents of calycosin, formononetin and astragalosideⅣ, Ⅱ , Ⅲ among 15 batches from different habitats. Methods： HPLC -DAD -ELSD was conducted on a SpursilTMcls column（5 μm,250 mm ×X 4.6 ram）with gradient elution , the mobile phase was ACN and H2 O, the temperature of drift tube was 112. 8℃ and the nebulizing gas flow rate was 3.2 L · rain^-1. Results： Five compounds were simul- taneously detected with one injection,good linear relationships were found（r =0. 9992 -0. 9999） ,and the average recovery rates were 99. 13% - 100.94%. Conclusions：The HPLC - DAD - ELSD method is simple and reproduci- ble, which can be used as principles of quantitative analysis.

关 键 词：黄芪 高效液相色谱-二极管阵列检测器-蒸发光散射检测器 毛蕊异黄酮 芒柄花素 黄芪甲苷(黄芪皂苷Ⅳ) 黄芪皂苷Ⅱ 黄芪皂苷Ⅲ 含量测定 

分 类 号：R917[医药卫生—药物分析学]