HPLC-ESI-MS和HPLC法分析测定灵芝醇提物中3种灵芝酸  

Determination of three ganoderic acids in Lingzhi ethanol extracts by HPLC-ESI-MS and HPLC

作　　者：王勇[1] 陈硕[1] 卢端萍[1] 蒋婷婷[2] 范明[1] 

机构地区：[1]福建省药品检验所,福州350001 [2]福建医科大学药学院,福州350108

出　　处：《药物分析杂志》2013年第2期292-298,321,共8页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:对灵芝醇提物中灵芝酸A、B、C2的含量进行测定,为灵芝醇提物的质量控制提供依据。方法:采用高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)法对灵芝醇提物中的灵芝酸A、B、C2进行定性分析,采用HPLC法对3种灵芝酸的含量进行测定。结果:HPLC-ESI-MS法测定结果显示,灵芝醇提物含有灵芝酸A、B、C2。HPLC法测定结果显示,灵芝酸A、B、C2分别在3.50～525.60μg.mL-1(r=1.0000)、3.23～484.80μg.mL-1(r=1.0000)、2.04～203.6μg.mL-1(r=0.9999)浓度范围内呈现良好的线性。平均回收率分别为98.2%,101.5%,98.7%(n=9)。不同来源的赤芝、紫芝醇提物中灵芝酸A、B、C2总含量差异较大,灵芝酸A在3种灵芝酸中含量最高,紫芝醇提物中灵芝酸A、B、C2的总含量较赤芝醇提物的低。结论:该方法稳定性、耐用性良好,可用于灵芝醇提物的质量控制。Objective ：To determinate the contents of ganoderic acids A, B, C2 in Lingzhi ethanol extracts, so as to provide a basis for quality control of Lingzhi ethanol extracts. Methods：The methods of HPLC - ESI - MS and HPLC were adopted for qualitative and quantitive analyses of ganoderic acids A, B, C2 in Lingzhi ethanol extracts. Results： The HPLC - ESI - MS analysis showed that Lingzhi ethanol extracts contained ganoderic acid A, B, C2. The HPLC analysis showed that the method had good linearity for ganoderic acid A, B, C2 in the range of 3.50 - 525.60 μg · mL^-1 （r = 1.0000）,3.23 -484.80 μg · mL^ -1（r = 1.0000）,2.04 -203.6 μg · mL^-1 （r = 0. 9999） , respectively. The average recoveries of ganoderic acid A, B, C2 were 98.2% , 101.5% ,98.7% （ n = 9 ） , respectively. The contents of total ganoderic acids were different in the same species and the ganoderic acid A was the major compound of the three ganoderie acids. The total contents in G. sinensis ethanol extracts were far lower than those in the G. lucidum. Conclusion：The methods are stable and durability, which can be used for the quality control of Lingzhi ethanol extracts.

关 键 词：灵芝 赤芝 紫芝 补益药物 三萜类化合物 灵芝醇提物 灵芝酸 高效液相色谱-电喷雾-质谱 

分 类 号：R917[医药卫生—药物分析学]