银杏叶提取物对大鼠视神经夹伤后视神经保护作用的研究  

Neuroprotective effect of ginkgo biloba extract EGb761 in the rat after optic nerve crush

作　　者：孙海燕[1] 卞晓芸[1] 吴香丽[1] 刘晓坤[1] 张筠[1] 闫志鹏[1] 

机构地区：[1]河北医科大学第三医院眼科,河北石家庄050000

出　　处：《中华眼外伤职业眼病杂志》2013年第2期81-85,共5页Chinese Journal of Ocular Trauma and Occupational Eye Disease

摘　　要：目的探讨银杏叶提取物（EGb761）对大鼠视神经钳夹伤后视神经的保护作用。方法60只大鼠随机分为3组：正常组、对照组、治疗组，对照组及治疗组制备视神经损伤的动物模型，治疗组于伤后1h开始给予EGb7610．05ml（100mg／kg）球后注射，隔日1次。对照组给予等量的生理盐水。在伤后3d、7d、14d、28d取材，采用苏木精伊红（HE）染色观察各组视神经组织形态学变化、免疫组织化学测定各组视神经诱导型一氧化氮合成酶（iNOS）、B细胞淋巴瘤／白血病．2（Bcl-2）、Bcl-2相关x蛋白（BAX）阳性细胞和Bcl-2／Bax的变化。结果3d、7d、14d及28d，对照组和治疗组iNOs阳性细胞表达较正常组显著升高（P〈0．01）；治疗组较对照组iNOs阳性细胞表达显著降低（P〈0．01）；差别均有统计学意义。3d、7d、14d及28d治疗组Bcl-2阳性细胞较对照组显著升高（P〈0．01），差异均有统计学意义。3d、7d治疗组Bax阳性细胞较对照组显著降低（P〈0．01），14、28d治疗组与对照组无明显差别（P〉0．05）。结论EGb761能降低视神经组织iNOs、Bax蛋白的表达，增加Bcl-2蛋白表达，提高Bcl-2／Bax的比率，抑制RGCs细胞凋亡，对视神经有保护作用。Objective To explore the neuroprotective effect of ginkgo biloba extract EGb761 in the rat after optic nerve crush. Methods Sixty SD rats were randomly divided into three groups：untreated,con- trol and treated group. The untreated group had no treatment. The right eyes of the rats in the control and treated group were made into the optic nerve crush model. For the treated group, EGb761 （0.05 ml, 100 mg/ kg） was injected retrobulbarly at 1 b after the injury once every other day. Normal saline was injected in the control group in the same way. The rats were euthanatized 3,7,14 and 28 days after crush. The optic nerve was stained by hematoxylin-eosin to show the morphological changes. The changes of inducible nitric oxide synthase （iNOS）, B cell lymphoma/leukemia-2 （Bcl-2）, Bcl-2 associated X protein （BAX） positive cells and Bcl-2/Bax were tested by immunohistoehemistry. Results The expression levels of positive iNOS cells in the control group and the treated group were higher than that in the normal group（P 〈 0.01 ） on 3,7,14, and 28 day;and the treated group has lower positive iNOS rate than that in the control group（P 〈 0.01 ）. The differences were statistically significant. The expression level of positive Bcl-2 cells in the treated group was significantly higher than that in the control group（ P 〈 0.01 ） on day 3,7,14, and day 28. The expression lev- el of positive BAX cells in the treated group was significant lower than that in the control group（ P 〈 0.01 ）on day 3,7. However there were no significant differences between the treated and control group at day 14,28. Conclusion Ginkgo biloba extract EGb761 has neuroprotective effect in the rat after optic nerve crush thr-ough reducing the expression of iNOS and BAX,increasing the expression of Bcl-2, elevating Bcl-2/Bax and suppressing the apoptosis of retinal ganglion cells （RGCs）.

关 键 词：视神经 银杏叶提取物 诱导型-氧化氮合成酶 B细胞淋巴瘤 白血病-2 Bcl-2相关X 蛋白 

分 类 号：R285.5[医药卫生—中药学]