天麻与钩藤配伍前后对SHR大鼠肝脏相关基因表达的影响  

Impact of the Compatibility of Gastrodia elata with Uncaria Ramulus Cum uncis on Liver Gene Expression in SHR Rats

作　　者：李晓倩[1] 王兴[1] 李莹[1] 袁琳[1] 张卫国[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《中国药房》2013年第7期580-582,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.30801547);中央高校基本科研业务会专项资金资助项目(No.SWJTU09ZT29)

摘　　要：目的:研究天麻与钩藤配伍前后对SHR大鼠肝脏相关基因表达的影响。方法:实验分为空白、天麻(2.3ml)、天麻钩藤(2.7ml)组,灌胃给药,每天1次,连续10d。末次给药后提取肾脏mRNA,与基因芯片进行杂交,经扫描、计算机软件分析后观察配伍前后基因表达的变化。结果:筛选出天麻与钩藤配伍前后在肝脏中的相关基因16个,主要集中在调控血脂代谢、炎症反应、G-蛋白偶联受体介导的信号通路以及胰岛素抵抗等方面。结论:天麻钩藤配伍后可增强天麻对肝脏的相关作用。OBJECTIVE： To study the impact of the compatibility of Gastrodia elata with Uncaria Ramulus Cum uncis on liver gene expression in SHR rats. METHODS： SHR rats were divided into 3 groups, i.e. blank group, G. clara（2.3 ml） group, G. elata and Uncaria Ramulus Cum uncis（2.7 ml） group. They were given medicine intragastrically once a day for consecutive 10 days. The mRNA in the kidney of SHR rats was extracted after the last medication, and then was hybridized with gene chip. After computer- ized scanning and analyzing, the changes of gene expression were observed before and after compatibility. RESULTS： 16 target genes were selected before and after compatibility of G. elata with Uncaria Ramulus Cum uncis, in field of regulating lipid metabo- lism, inflammatory reaction, G-protein coupled receptor mediated signal channel and insulin resistance. CONCLUSION： It indi- cates that the related effects of G. elata on liver are strengthened after the compatibility.

关 键 词：天麻 钩藤 配伍 肝脏 基因表达 

分 类 号：R285[医药卫生—中药学]