髓复康对大鼠脑缺血损伤区内髓鞘相关蛋白和硫酸软骨素蛋白多糖表达的影响  

Effects of Suifukang on the Expression of MAG and CSPGs in Cerebral Ischemic Injury Area of Rats

作　　者：张平[1] 胡佩岩[1] 李国辉[1] 

机构地区：[1]中国中医科学院望京医院,北京100102

出　　处：《中国药房》2013年第7期583-585,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.30701094)

摘　　要：目的:研究髓复康对大鼠脑缺血损伤区内髓鞘相关蛋白(MAG)和硫酸软骨素蛋白多糖(CSPGs)的影响。方法:通过Koizumi法复制SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常对照(等容生理盐水)、模型(等容生理盐水)、甲基强的松龙(30mg/kg)和髓复康高、中、低剂量(50、25、12.5g/kg)组,各组又分别在给药8、15、30d取大鼠脑组织,通过免疫组化法检测各组脑缺血损伤区MAG和CSPGs的表达。结果:在相应时间点,与模型组比较,髓复康高、中、低剂量组MAG和CSPGs表达显著减弱(P<0.01或P<0.05)。结论:髓复康可以抑制脑缺血损伤区MAG和CSPGs的表达,通过改善脑缺血损伤区轴突再生的微环境,促进脑缺血损伤后轴突的再生。OBJECTIVE： To study the inhibitory effect of Suifukang on the expression of chondroitin sulfate proteoglycans （CSPGs） and myelin-associated glycoprotein （MAG） in cerebral ischemic injury area of rats. METHODS： Middle cerebral artery occlusion （MACO） model of SD rats were induced and randomized into normal group （isovolumic saline）, model group （isovolu- mic saline）,methylprednisolone group（30 mg/kg）, Suifukang high-dose, medium-dose and low-dose groups（50,25,12.5 g/kg）. Ce- rebral tissues of rats were collected after 8 d, 15 d and 30 d treatment. The expressions of CSPGs and MAG were detected by im- munohistochemistry assay. RESULTS： Compared with model group, the expressions of CSPGs and MAG in Suifukang high-dose, medium-dose and low-dose groups were less （P〈0.01 or P〈0.05）in the corresponding time point. CONCLUSION： Suifukang can inhibit the expression of CSPGs and MAG, and improve the microenvironment of axon regeneration in cerebral ischemic injury area so as to promote axon regeneration after cerebral ischemic injury.

关 键 词：髓复康 脑缺血损伤 硫酸软骨素蛋白多糖 髓鞘相关蛋白 

分 类 号：R285[医药卫生—中药学] R283[医药卫生—中医学]