黄柏粗提物及其含药血清对K562/A02细胞P-gp表达和活性的影响  

Effect of Crude Extract of Phellodendron chinese and Drug Serum on the P-gp Expression and Activity of K562/A02 Cells

作　　者：徐宋瑶[1] 王春梅[1] 刘华凤[1] 

机构地区：[1]北京中医药大学中药学院生物制药系,北京100102

出　　处：《中国药房》2013年第7期586-588,共3页China Pharmacy

基　　金：国家重点基础研究发展计划资助(No.2007CB512605)

摘　　要：目的:研究黄柏粗提物及其含药血清对K562/A02细胞P-糖蛋白(gp)的影响。方法:用黄柏粗提物(2、1mg/ml)及含药血清(10%)培养K562/A02细胞,收集细胞提取蛋白,以Western blot法检测P-gp的表达;以流式细胞仪测定罗丹明123的泵出检测P-gp的活性。结果:高、低浓度黄柏粗提物作用于K562/A02细胞后,P-gp表达量和活性与空白血清组比较无显著性差异;黄柏含药血清作用于K562/A02细胞后,P-gp表达量和活性降低。结论:黄柏含药血清可抑制P-gp的活性和表达。OBJECTIVE ： To explore the effect of crude extract of Phellodendron on chinese and drug serum on the P-gp expres- sion and activity. METHODS ： The crude extracts of P. chinese or drug serum were used to treat the K562/A02 cells. The P-gp pro- tein expression level was detected by Western blot and the pump output of Rho123 were measured by flow cytometry to determine P-gp activity of the cells. RESULTS： After treated with high concentration, low concentration crude extracts of B chinese, the P-gp protein expression or its activity had no significant difference. The serum containing P. chinese decreased the P-gp protein expres- sion and its activity. CONCLUSION： The serum containing P. chinese could inhibit P-gp expression and activity.

关 键 词：黄柏 含药血清 P-糖蛋白 

分 类 号：R285[医药卫生—中药学] R282.75[医药卫生—中医学]