魟鱼软骨多糖对小鼠黑色素瘤上皮细胞激酶表达的影响  

Effect of RCG on the Expression of EphA2 in Melanoma of Mice

作　　者：王冠[1] 郭斌[2] 孟康[1] 

机构地区：[1]辽宁医学院药学院,辽宁锦州121001 [2]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《中国药房》2013年第7期589-591,共3页China Pharmacy

基　　金：辽宁省自然科学基金资助项目(No.201102132)

摘　　要：目的:研究魟鱼软骨多糖(RCG)对小鼠黑色素瘤中上皮细胞激酶(EphA2)表达的影响。方法:将培养的黑色素瘤细胞接种于C57BL/6小鼠右侧腋下部位,复制小鼠黑色素瘤模型。实验分为模型(等容生理盐水)、环磷酰胺(CTX,60mg/kg)与RCG高、中、低剂量(700、300、100mg/kg)组,观察肿瘤生长情况,计算抑瘤率;采用Western blot法检测EphA2蛋白的表达,采用RT-PCR法检测EphA2基因的表达。结果:与模型组比较,RCG高、中、低剂量组小鼠瘤质量显著减轻,抑瘤率显著升高(P<0.01);EphA2蛋白表达显著减弱(P<0.01),EphA2基因表达显著减弱(P<0.01)。结论:RCG抑制小鼠黑色素瘤的生长可能与其减少肿瘤中EphA2表达有关。OBJECTIVE： To study the effect of RCG on the protein expression of epithelial cell kinase （EphA2） in melanoma of mice. METHODS：Melanoma tumor cells were cultivated in vitro, then inoculated in right oxter of C57BL/6 mice. The malignant melanoma model was established. The experimental mice were randomly divided into model group（isovolumic saline）, CTX group （60 mg/kg） and RCG high-dose, medium-dose and low-dose groups （700, 300, 100 mg/kg）. The growth of tumor was observed and the inhibitory rate of tumor was measured; the protein expression of EphA2 was determined by Western blot, and the mRNA expression of EphA2 was detected with RT-PCR. RESULTS： Compared with model group, tumor weight were lost, inhibitory rates of primary tumor in RCG groups were increased significantly（P〈0.01） ; the protein expression of EphA2 and the gene expression of EphA2 in RCG groups were lowered significantly（P〈0.01）. CONCLUSION： RCG could inhibit the growth of melanoma of mice, which is probably associated with that RCG reduce the protein expression of EphA2 in the tumor cells.

关 键 词：魟鱼软骨多糖 恶性黑色素移植瘤 上皮细胞激酶 

分 类 号：R285[医药卫生—中药学] R282.74[医药卫生—中医学]