连朴饮传统煎剂与中药配方颗粒中盐酸小檗碱的含量比较  

Comparison of the Contents of Berberine Hydrochloride in Traditional Decoctions vs.TCM Formula Granules of Lianpu Drink

作　　者：罗秀琼 冯华 

机构地区：[1]遵义市食品药品检验所,贵州遵义563002

出　　处：《中国药房》2013年第7期637-639,共3页China Pharmacy

基　　金：遵义市中药现代化科技产业研究开发专项项目(市科合中药专字〔2009〕4号)资助

摘　　要：目的:建立连朴饮传统煎剂(合煎剂)与中药配方颗粒(分煎剂)中盐酸小檗碱的含量测定方法并比较二者含量。方法:采用高效液相色谱法。色谱柱为Diamonisl C18(200mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(50:50,V/V)(每100ml加十二烷基磺酸钠0.1g),流速为1.0ml/min,检测波长为265nm。结果:盐酸小檗碱进样量在2.0～10.0μg范围内与峰面积积分值呈良好线性关系(r=0.9992);平均加样回收率为97.30%,RSD=1.22%(n=6)。结论:与传统煎剂比较,连朴饮中药配方颗粒中盐酸小檗碱的含量更高。OBJECTIVE： To establish the method for the content determination of berberine hydrochloride in traditional decoc- tion （mixed decoction） vs. TCM formula granules （separated decoction） of Lianpu drink, and to compare the contents of them. METHODS： HPLC method was adopted. The determination was performed on Diamonisl C18 column（200 mm×4.6 mm, 5 μm） with mobile phase consisted of acetonitrile-0.1% phosphoricacid solution （50：50, V/V）（0.1 g sodium xanthate dodecyl added into 100 ml solution）, at the flow rate of 1.0 ml/min; d＇etection wavelength was set at 265 nm. RESULTS： The linear range of berber- ine hydrochloride were 2.0-10.0μg（r=0.999 2） with average recovery of 97.30% （RSD= 1.22% ,n=6）. CONCLUSION： The con- tent of berberine hydrochloride in TCM formula granules of Lianpu drink is higher than in traditional decoction.

关 键 词：连朴饮 盐酸小檗碱 合煎 分煎 中药配方颗粒 高效液相色谱法 

分 类 号：R283[医药卫生—中药学] R927.2[医药卫生—中医学]