新疆梨树上苹果锈果类病毒的检测与全序列分析  

Detection and Sequence Analysis of the Apple Scar Skin Viroid Isolated from Pear Trees in Xinjiang

作　　者：王钰婷[1] 金珠[1] 董芳园[1] 和世玉[1] 张莉[1] 牛建新[1] 

机构地区：[1]石河子大学农学院,新疆石河子832003

出　　处：《新疆农业科学》2013年第1期94-99,共6页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30360066);国家科技攻关计划引导项目(2003BA546C);石河子大学自然科学与技术创新项目(ZRKX200707)

摘　　要：[目的]检测并分析新疆和静县223团场多年生早熟梨树上的苹果锈果类病毒(ASSVd)。[方法]对采自新疆和静县223团多年生早熟梨的枝条和叶片样品进行RNA抽提,经RT-PCR技术鉴定检测。[结果](1)多年生早熟梨树检测到苹果锈果类病毒,得到2条ASSVd核酸序列(JX861258～JX861259),所得序列与GenBank中已报道的国内外ASSVd序列同源性达86%以上。(2)原位RT-PCR检测进一步表明ASSVd的RNA阳性信号主要位于叶片肉组织的细胞核内。[结论]通过序列分析比对,各寄主上的ASSVd分离物核酸序列变异较小,地域和品种间核酸序列无明显差异。建立了优化的RT-PCR检测及原位RT-PCR检测方法,为果树ASSVd的快速检测奠定了良好的基础。[ Objective and Method ] In order to detect and analyze the ASSVd in precocious pear trees, low molecular weight RNAs were extracted from tender leaves and shoots of precocious pear trees which were collected from No. 223 Farming ＆ Herding Regiment in Hejing County, Xinjiang Uygur Autonomous Region. Then the samples were detected by reverse transcription -polymerase chain reaction （RT- PCR）. [ Result ] The results showed： （ 1 ） Two of the precocious pear trees were infected with Apple scar skin viroid （ASSVd） and the 2 isolates （ accession numbers JX861258 - JX861259 ） had over 86% nucleotide sequence identity with previously published sequences in the GenBank. （2） In situ RT - PCR further confirmed the existence of ASSVd in the leaf tissues and indicated that ASSVd was mainly distributed in the nucleus. [ Conclusion ] There was no geographic and variety differentiation between ASSVd isolates. Optimized detection methods of RT- PCR and In situ RT- PCR were established, thus providing the basis for the rapid identification of ASSVd in fruit trees.

关 键 词：梨树 苹果锈果类病毒(ASSVd) 检测 序列分析 

分 类 号：S436.612.1[农业科学—农业昆虫与害虫防治]