离子色谱在多糖疫苗及多糖蛋白结合疫苗质量控制中的应用  

The application of ion chromatography in quality control of polysaccharide vaccine and conjugate vaccine

作　　者：慕艳红[1] 王晶[1] 赵志强[1] 朱莉萍[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,兰州730046

出　　处：《微生物学免疫学进展》2012年第6期78-82,共5页Progress In Microbiology and Immunology

摘　　要：离子色谱具有方便、快速、灵敏度高、选择性好、重现性好、准确度高、可同时分析多种离子化合物等优点,其中的高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)可直接分析糖类物质,可应用于多糖疫苗及多糖蛋白结合疫苗中多糖的分析。近年来,离子色谱应用在多糖及多糖蛋白结合疫苗的质量控制中,如测定疫苗中的多糖、游离糖、杂质多糖、对人有免疫原性的功能基团等的研究有许多报道,对有关内容进行了综述。The ion chromatography(IC) can be used simultaneously and quickly in detection of several ionic compounds with high sensitivity,selectivity accuracy and reproducibility,especially,high performance anion exchange chromatography with pulsed amperometric detection(HPAEC-PAD)can be used in detection of carbohydrates directly,so it can be used in the quality control of polysaccharide vaccine and conjugate vaccine,including the determination content of total polysaccharide,free polysaccharide,and impurities in polysaccharide and some functional immunogenic epitopes in of humans.This review presents some progress in this aspect in recent years.

关 键 词：离子色谱 HPAEC-PAD 多糖疫苗 多糖蛋白结合疫苗 

分 类 号：R392[医药卫生—免疫学]