阻塞性睡眠呼吸暂停综合征大鼠皮质海马损害及机制研究  

Injury of cortex and hippocampus in rats with obstructive sleep apnea syndrome and its mechanisms

作　　者：宋凯英[1,2] 徐平[1] 史艳红[1] 

机构地区：[1]遵义医学院附属医院神经内科,563000 [2]河南省扶沟县人民医院神经内科

出　　处：《中华老年心脑血管病杂志》2013年第2期193-196,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金："天士力"杯脑血管病科研基金(TSL2011005)

摘　　要：目的通过建立大鼠阻塞性睡眠呼吸暂停综合征(OSAS)模型,探讨OSAS与缺血性脑卒中的相关性。方法清洁级Wistar大鼠20只,随机分为正常对照组和OSAS组,各10只。OSAS组大鼠咽腔多点注射透明质酸钠凝胶建立OSAS模型,并监测脑电、口鼻气流及血氧;检测2组血C反应蛋白(CRP)、纤维蛋白原(Fib)、同型半胱氨酸(Hcy)水平;HE染色法观察大鼠皮质及海马结构。结果 OSAS组大鼠CRP、Fib较正常对照组明显升高(P<0.05),2组Hcy水平比较,差异无统计学意义(P>0.05);CRP和Fib与平均血氧饱和度呈负相关(r=-0.802,-0.867,P<0.05),与睡眠呼吸暂停指数呈正相关(r=0.874,0.941,P<0.05)。OSAS组大鼠脑组织HE染色皮质及海马区有神经元缺失、排列紊乱及脑小血管增生。结论 OSAS大鼠CRP、Fib水平升高,皮质海马区神经元形态学发生变化,可能是OSAS发生缺血性脑卒中的发病机制。Objective To study the correlation between obstructive sleep apnea syndrome（OSAS） and ischemic stroke by establishing a rat OSAS model. Methods Twenty clear Wistar rats were randomly divided into control group and OSAS group（10 in each group）. An OSAS model was es- tablished by injectiing sodium hyaluronate gel into the rats of OSAS group. Nose and mouth air- flow, and dynamic oxygen in the rats were monitored by EEG. Their serum levels of C-reactive protein（CRP） ,fibrinogen（Fib） and homocysteine（Hcy） were measured. Structure of cortex and hippocampus was observed with HE staining. Results The serum CRP and Fib levels were signif- icantly higher in OSAS group than in control group （P 〈 0. 05）. No significant difference was found in the serum Hcy level between the two groups（P〉0.05）. The serum CRP and Fib levels were negatively correlated with the mean blood oxygen saturation and positively correlated with the sleep apnea index. Brain tissue HE staining showed loss and disarrangement of neurons and proliferation of brain small blood vessels in OSAS group. Conclusion The high serum CRP and Fib levels and morphological change of neurons in cortex and hippocampus are the pathogensis of ischemic stroke in OSAS rats.

关 键 词：睡眠呼吸暂停 阻塞性 大脑皮质 海马 C反应蛋白质 纤维蛋白质 高半胱氨酸 

分 类 号：R766[医药卫生—耳鼻咽喉科]