EV71临床分离株BALB/c小鼠致病特点分析  

Analysis of the pathogenic characteristics of BALB/c mice infected with enterovirus 71 isolates

作　　者：宋楠楠[1] 张颖[2] 李鹏[1] 岳盈盈[1] 李志会[1] 盖中涛[2] 孟红[1] 

机构地区：[1]山东省医学科学院基础医学研究所山东省罕少见病重点实验室,济南250062 [2]济南市传染病医院6科,济南250021

出　　处：《山东大学学报（医学版）》2012年第8期35-39,45,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81000720);山东省自然科学基金(ZR2011HQ030)

摘　　要：目的将肠道病毒71型(EV71)临床分离株接种小鼠观察其致病特点。方法将6株EV71临床株分别腹腔接种1日龄BALB/c小鼠,观察小鼠表现以及脑、肺脏、骨骼肌组织病理变化;采用免疫组化和RT-PCR方法,检测组织中病毒抗原和基因片段。结果临床分离的6株EV71(C4亚型)接种1日龄小鼠均出现病症,其中JN200804株实验小鼠症状较重,小鼠脑组织和骨骼肌可观察到细胞空泡变性、淋巴细胞浸润等病理变化;JN200804株感染组小鼠脑内检测到EV71抗原和基因片段。结论 6株EV71临床株均可感染小鼠并致病,重者出现松弛型麻痹并死亡,轻者出现震颤、麻痹但可恢复至正常。Objective To study the characteristics of enterovirus 71（EV71）isolates in vivo,and to provide information for the research of EV71.Methods One-day old BALB/c mice were infected with 6 EV71 strains isolates,and symptoms were observed.All mice were sacrificed at 7d after the infection to collect organs and tissues for the pathological examination,RT-PCR detection and immunohistochemistry.Results 6 viruses were isolated from stool samples,and identified as C4 genotype of EV71.All of them could induce lesions of one-day old mice.The JN200804 strain induced flaccid paralysis on mice 5 days after the infection.Histopathologically,lymphocytic infiltration and some other pathologic changes were observed in the infected mice brain,lung and skeletal muscles.mRNA and antigens was detected in the brain of mice infected with JN200804.Conclusion 6 strain of EV71 isolates can cause the disease on BALB/c mice,including the paralysis,muscular tremor,and death of infected mice.

关 键 词：肠道病毒C型 人 免疫组织化学 逆转录聚合酶链反应 基因 小鼠 近交BALB C 

分 类 号：R373.2[医药卫生—病原生物学]