冠心病患者外周血内皮祖细胞数量差异及其对内皮功能的影响  

Quantity variance of endothelial progenitor cells in peripheral blood of patients with coronary heart disease and its influence on endothelial function

作　　者：陈伟[1] 谈红[1] 李晓燕[1] 杨燕[1] 韦修莹[1] 陈英剑[1] 张红明[1] 

机构地区：[1]济南军区总医院心血管内科,济南250031

出　　处：《山东大学学报（医学版）》2012年第8期73-76,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2010HM202)

摘　　要：目的探讨冠心病患者外周血内皮祖细胞(EPCs)的水平及其对内皮功能的影响。方法随机选取经冠脉造影确诊为冠心病患者53例为冠心病组,非冠心病患者52例为正常对照组,采用超声测定其内皮舒张功能指标(FMD),采用流式细胞术检测各组EPCs水平,同时检测反映内皮功能的生化指标,采用免疫散射比浊法测定高敏C反应蛋白(hs-CRP)水平,硝酸盐还原酶法测定NO。结果在基线水平上,冠心病组内皮功能较正常对照组明显低下,EPCs数目明显减少,hs-CRP水平升高,NO含量减低,差异均有统计学意义(P均<0.05);FMD与EPCs数量显著相关,FMD随EPCs数量增加而改善,呈正相关趋势(r=0.52,P<0.01)。同时,反映内皮功能的指标hs-CRP与FMD、EPCs均呈负相关趋势(r=-0.43,r=-0.50,P<0.05),NO与FMD、EPCs均呈正相关趋势(r=0.54,r=0.45,P<0.05)。结论冠心病患者EPCs数量较健康人群明显减少,内皮功能明显低下,同时内皮功能随EPCs数量增加而改善。Objective To investigate the variance of endothelial progenitor cells（EPCs） in peripheral blood of patients with coronary heart disease,and its influence on endothelial function.Methods 53 patients with confirmed CHD by coronary angiography were recruited randomly as the CHD group,and 52 patients without CHD were chosen as the control group.Humeral flow-mediated-dilation（FMD） was measured with high resolution ultrasonography for each patient.The levels of EPCs were detected in both groups by flow cytometry.The plasma levels of nitric oxide（NO） and high sensitivity C-reactive protein（hs-CRP） were also assessed in both groups.Results At the baseline,compared with those people without CHD,the endothelial function of patients with CHD was significantly lower,and the number of EPCs was significantly reduced,hs-CRP significantly increased,NO was significantly reduced（P0.05）;There was a significant positive relationship between endothelial function and the number of circulating EPCs,endothelial function improved with the increasing of the number of EPCs（r=0.52,P0.01）.Meanwhile,hs-CRP,one of the indices reflecting endothelial function,was negatively correlated with FMD and EPCs（r=-0.43,r=-0.50,P0.05）,NO was positively related with FMD and EPCs（r=0.54,r=0.45,P0.05）.Conclusion In patients with CHD,there is endothelial dysfunction,and the number of EPCs is significantly reduced.The endothelial function improves with the increasing of EPCs number.

关 键 词：冠心病 内皮祖细胞 内皮功能 高敏C反应蛋白 一氧化氮 

分 类 号：R541.4[医药卫生—心血管疾病]