乙型肝炎病毒基因组1896位点变异株与野生株的定性检测  

The qualitative detection of hepatitis B virus genome 1896 site variants and wild strains

作　　者：郭龙华 陈茶[2] 万泽民[2] 何文军[2] 吴新忠[2] 周强[2] 冯春颜 吴文权 

机构地区：[1]深圳市龙华人民医院检验科,广东深圳518109 [2]广东省中医院检验科,广州510120

出　　处：《重庆医学》2013年第3期308-309,共2页Chongqing medicine

基　　金：广东省科技计划项目(2009B030801289)

摘　　要：目的建立一种能同时检测乙型肝炎病毒(HBV)基因组1896位点变异株与野生株的定性方法。方法设计3条特异性引物(组成2对引物),提取血清中HBV DNA模板进行PCR扩增变异株与野生株,取PCR产物在琼脂凝胶(含EB)上电泳,观察结果。结果本次实验共检测40个标本,有11个标本只检测到野生株,8个标本只检测到变异株,其余21个标本都是变异株与野生株混合感染。结论该方法是一种可行的能同时检测HBV基因组1896位点变异株与野生株的定性方法。Objective To establish the qualitative detection method of hepatitis B virus（HBV） genome 1896 site variants and wild strains.Methods 3 strips of specific primers（consisting of 2 pairs of primers） were designed.HBV DNA templates extracted from the serum samples were amplified by PCR using 2 pairs of primers against variant strains and wild strains,and then we observed PCR products by agar gel electrophoresis.Results The experiments detected 40 samples,wild strains were detected in 11 samples,variant strains were detected in 8 samples.Variant and wild strains of mixed infection were detected in the remaining 21 samples.Conclusion This is a feasible method to detect HBV genome 1896 site variant and wild strains of qualitative methods.

关 键 词：乙型肝炎病毒 野生株 变异株 

分 类 号：R512.62[医药卫生—内科学]