金属硫蛋白和黄酮对急性铅中毒大鼠代谢的研究  

Metabolic study in acute lead-treated rats by metallothionein and flavone

作　　者：廖丹[1] 丁世彬[1] 龙甲[1] 金鑫[1] 兰泽桥 李义勇 文左凤 曹玉广[1] 应晨江[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系 [2]武汉天峡鲟都科技有限公司,武汉430030

出　　处：《微量元素与健康研究》2013年第1期1-3,11,共4页Studies of Trace Elements and Health

基　　金：国家自然科学基金资助项目(30972473);天峡鲟龙股份有限公司资助

摘　　要：目的:探讨金属硫蛋白和黄酮对急性高剂量染铅大鼠代谢的影响。方法:25只Wistar大鼠随机分为MT组、黄酮组和依地酸二钠钙组(给予醋酸铅后再分别给予MT、黄酮和依地酸二钠钙)、染铅模型组和对照组。采集血液、股骨、粪便和尿液,分别用原子吸收分光光度法测定铅含量。结果:与对照组比较,染铅模型组大鼠血铅、股骨铅、粪铅及尿铅含量均显著升高(P<0.05)。与染铅模型组比较,MT组、黄酮组和依地酸二钠钙组血铅、股骨铅含量均下降(P<0.05),粪便排铅量明显增加(P<0.05);与依地酸二钠钙组比较,MT组和黄酮组尿液排铅量明显减少(P<0.05)。MT组和黄酮组大鼠血清ALT活力明显低于染铅模型组(P<0.05)。结论:MT和黄酮能够明显促进急性铅中毒大鼠排铅,主要通过粪便途径,且对肝脏有一定的保护作用。Objective： To observe the effect of metallothionein（MT） and flavone on metabolism in acute lead-treated rats.Methods： A total of 25 Wistar rats were randomly divided into five groups： lead-exposure with MT-treated,lead-exposure with flavone-treated and lead-exposure with EDTA-treated groups,lead-exposure,and normal control.Then all the rats were placed in the metabolic cage.Urine and feces were collected over 24 h after administration from each rat.Leads in the whole blood,urine,feces and femur were measured by atomic absorption spectroscopy.Results： As compared with controls,the lead content of samples were significantly elevated in lead-treated rats（P0.05）.Compared with the lead-treated rats,MT,flavone and EDTA increased the blood,femur,faeces lead content（P0.05）.Both MT and flavone significantly decreased the urine lead content compared with EDTA groups,（P0.05）.The serum ALT in rats of MT and flavone treatment groups was significantly lower than lead-poisoned model group（P0.05）.The activity of AST and ACE in blood serum of all groups showed no difference.Conclusions： MT and flavone can obviously reduce the lead concentration and increase the expelling lead mainly via faeces,and showed liver protection.

关 键 词：金属硫蛋白 黄酮 急性铅中毒 代谢 

分 类 号：O612.4[理学—无机化学]