外源性八肽胆囊收缩素对吗啡所致SH-SY5Y和PC12细胞毒的保护作用  

CCK-8 PROTECTS MORPHINE-INDUCED CYTOTOXICITY IN SH-SY5Y AND PC12 CELLS

作　　者：李冬[1] 孟雁欣[1] 杨胜昌[1] 文迪[1] 丛斌[1] 马春玲[1] 

机构地区：[1]河北医科大学基础医学院法医学系,河北省法医学重点实验室,河北石家庄050017

出　　处：《河北医科大学学报》2013年第1期1-4,F0002,共5页Journal of Hebei Medical University

基　　金：国家自然科学基金(30672355;81172900);河北省应用基础研究重点基础研究项目(10966911D)

摘　　要：目的观察外源性八肽胆囊收缩素(cholecystokinin octapeptide,CCK-8)对吗啡处理SH-SY5Y及PC12细胞毒性作用的影响。方法应用二甲基噻唑二苯基四噻唑盐[3-(4,5)-dimethylthiazo(-2-yl)-3,5-diphenytetrazoliumromide,MTT]法观察吗啡对SH-SY5Y及PC12细胞毒性作用的剂量和时间依赖关系,并且观测外源性CCK-8对吗啡细胞毒性作用的影响。结果①吗啡在100～10 000μmol/L浓度范围内显著降低SH-SY5Y和PC12细胞的生存率,半数抑制率分别为2 520μmol/L和680μmol/L;上述作用在孵育48h明显,随时间延长逐渐增强;CCK-8(10^(-6)和10^(-8)mol/L)可明显抑制3 000μmol/L吗啡作用48h后SH-SY5Y细胞生存率的下降,CCK-8(10^(-6)、10^(-8)和10^(-10)mol/L)可明显抑制1 000μmol/L吗啡作用48h后PC12细胞生存率的下降;②3 000μmol/L和1000μmol/L吗啡分别处理SH-SY5Y细胞和PC12细胞48h后,可见细胞胞体变圆,突触变短或消失,失去原有的细胞形态。CCK-8(10^(-6)和10^(-8)mol/L)可明显改善吗啡作用后SH-SY5Y和PC12细胞的形态改变。结论外源性CCK-8可浓度依赖地对抗吗啡产生的细胞毒性作用。Objective To explore the effect of exogenous cholecystokinin octapeptide （CCK-8） on morphine-induced cytotoxicity. Methods 3-（ 4, 5 ） -dimethy hhiazo （ -2-yl ） -3, 5-di- phenytetrazoliumromide （MTT） assay was used to assess the dose-and time-dependent response of morphine-induced cytotoxicity in SH-SYSY and PC12 cells, also the effect of CCK-8 on morphine-induced cytotoxicity was observed. Results ① The concentration of morphine was from 100~mol/L to 10 000μmol/L which reduced the survival rate of SH-SYSY and PC12 cells significantly. And the half maximal inhibitory concentration was 2 520 μmol/L and 680μmoL/L respectively, meanwhile this role became obviously after 48h and then gradually increased with time. CCK-8 （ 10 -6 and 10 -Stool/L） could obviously inhibit the effect of morphine on the survival rate of SH-SY5Y cells ,while CCK-8 （10-6,10-s and 10-μmol/L） could obviously inhibit the effect of morphine on PC12 cells. ②After treating SH-SYSY cells and PC12 cells with 3 000μmol/L and 1 000μmol/L morphine separately for 48 hours, both of the cells lost their normal shape with synapse shorten or lost, CCK-8 （10-6 and 10-Smol/L） could significantly improve cell morphological alternation induced by morphine. Conclusion Exogenous CCK-8 could inhibit morphine-induced cytotoxicity in a concentration-dependent way.

关 键 词：受体 胆囊收缩素 吗啡 法医毒理学 

分 类 号：R894.2[医药卫生—法医学]