紫杉醇脂质体制备及体外抑制两种细胞的作用  被引量:2

Study on the Preparation and Inhibitory Effect on SKOV-3 and HUVEC Cells in vitro of Paclitaxel Liposomes

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作  者:符旭东[1] 胡戴[1,2] 李欧[1,2] 

机构地区:[1]广州军区武汉总医院药剂科,武汉430070 [2]湖北中医药大学

出  处:《中国药师》2013年第1期3-6,共4页China Pharmacist

基  金:2011年湖北省科技计划自然科学基金项目(编号:2011CDB019)

摘  要:目的:制备紫杉醇脂质体并考察体外对人卵巢癌上皮细胞(SKOV-3)和人脐静脉内皮细胞(HUVEC)两种细胞的抑制作用。方法:采用薄膜分散法制备紫杉醇脂质体,透射电镜观察脂质体的形貌,激光粒度仪测量粒径和Zeta电位,MTT法检测脂质体体外对SKOV-3和HUVEC的抑制作用。结果:制备的脂质体呈圆形或类圆形,平均粒径为54.6 nm,Zeta电位为-40.9 mv,体外释放符合Higuchi方程。体外细胞结果实验显示,紫杉醇能同时抑制肿瘤细胞和血管内皮细胞的生长。结论:紫杉醇脂质体较紫杉醇注射液(TAXOL)能更有效的抑制SKOV-3和HUVEC的生长。Objective:To prepare paclitaxel liposomes and study the inhibitory effect on SKOV-3 and HUVEC in vitrd.Method: Paclitaxel liposomes were prepared by film dispersion method.TEM was used to observe the morphology of the liposomes,and the particle size and potential were measured by a laser particle size analyzer.MTT experiment was adopted to evaluate the inhibitory effect of paclitaxel liposomes on SKOV-3 and HUVEC cells.Result;Paclitaxel liposomes showed round or oval morphology with the average particle size of 54.6 nm and Zeta potential of -40.9 mv.In vitro drug release was conformed to a Higuchi equation.The results of in vitro experiments suggested that paclitaxel liposomes had the ability to inhibit the growth of tumor cells and vascular endothelial cells. Conclusion:Paclitaxel liposomes possess higher inhibitory effect on SKOV-3 and HUVEC than paclitaxel injections(TAXOL).

关 键 词:紫杉醇 脂质体 血管内皮细胞 

分 类 号:R944.9[医药卫生—药剂学] R965.1[医药卫生—药学]

 

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