GC法测定维吾尔药红花籽和红花籽油中亚油酸的含量  

Determination of Linoleic Acid in Uygur Medicine Safflower Seed and Safflower Oil by GC

作　　者：王书云[1] 李国玉[2] 黄健[1] 王航宇[2] 张珂[2] 王金辉[1,2] 

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]石河子大学药学院

出　　处：《中国药师》2013年第1期13-16,共4页China Pharmacist

基　　金：国家科技支撑计划(编号:2012BAI30B02);石河子大学科学技术研究发展计划(编号:ZRKX2009ZD05);2010年国家药品标准提高项目(维吾尔药红花籽和红花籽油质量标准提高研究)

摘　　要：目的:建立GC内标法测定维吾尔药红花籽及红花籽油中亚油酸的含量。方法:色谱柱:TM-WAX柱(30 m×0.53 mm,1.0μm),流动相为氮气(纯度99.99%),流速:0.5 ml·min^(-1),分流比:5:1。柱温:185℃;进样口温度:250℃;FID检测器温度为280℃;流速:空气400 ml·min^(-1),氢气60.0 ml·min^(-1)。结果:亚油酸进样量在0.199～3.970μg范围内线性关系良好,r=0.999 9(n=6)。红花籽油的平均加样回收率为100.5%,RSD为1.24%;红花籽的平均加样回收率为101.9%,RSD为1.21%。结论:测定方法准确可靠,重复性好,可用于维吾尔药红花籽及红花籽油的定量测定。Objective：To develop a GC method with an internal standard for the determination of linoleic acid in Uygur medicine safflower seed and safflower oil.Method：The sample was separated on a TM-WAX（30 m×0.53 mm,1.0μm）column.The purity of nitrogen was 99.99%.The flow rate was 0.5 ml·min^-1 and the split ratio was 5：1.The column temperature was 185℃,the injection temperature was 250℃and the FID detector temperature was 280℃.The flow rate of air and hydrogen was 400 ml·min^-1 and 60.0 ml ？ min^（-1）,respectively.Result：The linear range of linoleic acid in safflower seed and safflower oil was 0.199-3.970μg（r =0.999 9, n = 6）.The average recovery of linoleic acid in safflower oil was 100.5%with RSD of 1.24%.The average recovery of linoleic acid in safflower seed was 101.9%with RSD of 1.21%.Conclusion：The method is accurate,convenient and reproducible in the determination of linoleic acid in Uygur medicine safflower seed and safflower oil.

关 键 词：红花籽 红花籽油 亚油酸 气相色谱法 内标法 

分 类 号：R927.2[医药卫生—药学]