不同炮制方法对黄连5种生物碱含量的影响研究  

Influences of Different Processing Methods on the Content of 5 Alkaloids in Rhizoma Coptis

作　　者：张帅[1] 赵宏冰[2] 何芳[3] 杨岩涛[2] 石继连[2] 

机构地区：[1]湖南中医药大学附属宁乡医院,湖南宁乡410600 [2]湖南中医药大学药学院 [3]湖南中医药大学人文社科学院

出　　处：《中国药师》2013年第1期19-21,共3页China Pharmacist

基　　金：湖南省自然科学基金项目(编号:12JJ3091);湖南省"十二五"重点学科中药学专业(编号:1008)

摘　　要：目的:比较黄连不同炮制品中5种生物碱的含量差异。方法:采用高效液相色谱法测定含量,色谱柱为Ultimate XB-C_(18)色谱柱(250 mm×4.6 mm,0.45μm);流动相:乙腈-(磷酸-三乙胺-水,1:2:100)(26:74);流速:1.0 rnl·min^(-1);检测波长:265 nm;柱温:30℃。结果:黄连各炮制品中的盐酸药根碱含量为0.36%～0.41%,其中姜黄连>萸黄连>酒黄连>生黄连:盐酸表小檗碱含量为0.91%～1.06%,其中萸黄连>姜黄连>生黄连>酒黄连;盐酸黄连碱含量为1.81%～2.09%,其中生黄连>酒黄连>萸黄连>姜黄连;盐酸巴马汀含量为1.42%～1.72%,其中萸黄连>生黄连>酒黄连>姜黄连;盐酸小檗碱含量为6.74%～7.12%,其中姜黄连>萸黄连>生黄连>酒黄连。结论:不同炮制方法对黄连5种生物碱含量均有不同的影响,为进一步探讨黄连的炮制机制提供试验基础。Objective：To compare the content difference of five alkaloids in rhizoma coptidis with different processing methods. Method：An HPLC was used to determine the alkaloids with an Ultimate XB-C_（18）（250 mm×4.6 mm,0.45μm） column.The mobile phase was acetonitrile-（phosphoric acid-triethylamine-water,1：2：100）（26：74）,the flow rate was 1 ml？ min^（-1）,the detection wavelength was 265 nm,and the column temperature was 301.Result：In the different processed products of rhizoma coptis,the content of jatrorrhizine hydrochloride was 0.36%-0.41%with the order of the product with ginger the product with fructus evodiae the product with yellow wine the crude.The content of epiberberine hydrochloride was 0.91%-1.06%with the order of the product with fructus evodiae the product with ginger the crude the product with yellow wine.The content of coptisine hydrochloride was 1.81%-2.09%with the order of the crude the product with yellow wine the product with fructus evodiae the product with ginger. The content of palmatine hydrochloride was 1.42%-1.72%with the order of the product with fructus evodiae the crude the product with yellow wine the product with ginger.The content of berberine hydrochloride was 6.74%-7.12%with the order of the product with ginger the product with fructus evodiae the crude the product with yellow wine.Conclusion：Different processing methods show different effects on the contents of the 5 alkaloids in rhizoma coptis,and the study can provide the experimental basis for the further study on the processing mechanism of rhizoma coptis.

关 键 词：黄连 炮制品 生物碱 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]