HPLC法测定复方咪康唑散中硝酸咪康唑含量  

Determination of Miconazole Nitrate in Compound Miconazole Powders by HPLC

作　　者：倪晓霞[1,2] 黄艺蓉[1,2] 黄元成[1,2] 田文君[1,2] 

机构地区：[1]解放军第175医院 [2]厦门大学附属东南医院,福建漳州363000

出　　处：《中国药师》2013年第1期82-84,共3页China Pharmacist

摘　　要：目的:建立高效液相色谱法测定复方咪康唑散中硝酸咪康唑的含量。方法:色谱柱:Angilent Eclipse XDB-C_(18)柱(150nn×4.6 mm,5μm),流动相:甲醇-乙腈-0.5%乙酸铵(42.5:42.5:15),检测波长:230 nm,流速:1.0 ml·min^(-1),柱温:30℃。结果:硝酸咪康唑在0.010 8～1.080 0 mg·ml^(-1)浓度范围内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为100.8%(RSD=1.05%,n=9)。结论:该法准确、重复性好、简便,可用于复方咪康唑散中硝酸咪康唑的含量测定。Objective：To establish an HPLC method for the determination of miconazole nitrate in compound miconazole powders. Method：The analytical column was an Agilent Eclipse XDB-C_（18）（150 mm×4.6 mm,5μm） column,methanol-acetonirile-0.5%ammonium acetate solution（42.5：42.5：15） was used as the mobile phase with the flow rate of 1.0 ml ？ min^（-1）,the detection wavelength was 230 nm and the column temperature was 30℃.Result：The calibration curve was linear within the concentration range of 0.010 8- 1.080 0 mg ？ ml^（-1）（r =0.999 9）,and the average recovery was 100.8%（RSD = 1.05%,n = 9）.Conclusion：The HPLC method is simple,accurate and reproducible,and can be used in the content determination of compound miconazole powders.

关 键 词：高效液相色谱法 复方咪康唑散 硝酸咪康唑 

分 类 号：R927.2[医药卫生—药学]