2型糖尿病大鼠肝脏损伤与固醇调节元件结合蛋白-1c、c-Jun氨基末端激酶表达的关系  

Relationship between the type 2 diabetic rat liver injury and the expression levels of SREBP-1c and JNK

作　　者：金磊[1] 刘立杰[2] 李慧[3] 

机构地区：[1]中国中医科学院广安门医院糖尿病实验室,北京100053 [2]北京中医药大学东方学院中医系教研室,河北廊坊065001 [3]北京大学糖尿病中心北京大学人民医院内分泌代谢科,北京100044

出　　处：《中国医药导报》2013年第5期19-21,共3页China Medical Herald

基　　金：国家自然科学基金资助项目(编号:81102484)

摘　　要：目的探讨固醇调节元件结合蛋白-1c(SREBP-1c)和c-Jun氨基末端激酶(JNK)在2型糖尿病大鼠肝损伤中的表达和意义。方法高脂饲料喂养结合小剂量链脲菌素建立2型糖尿病伴肝损伤大鼠模型组,同时以普食喂养的大鼠作为对照组,检测两组大鼠血糖、血脂水平,同时检测SREBP-1c及JNK的蛋白表达水平,观察大鼠肝损伤与SREBP-1c及JNK蛋白表达水平之间的关系。结果模型组血糖及血脂水平明显高于对照组,模型组肝功能生化指标检测显示损伤明显,模型组与对照组差异有统计学意义(P<0.05);模型组SREBP-1c蛋白表达阳性着色区域积分吸光度值为(51 767.21±3 246.37),JNK阳性表达率为90.9%;对照组阳性着色区域积分吸光度值为(35 814.37±2 971.52),JNK阳性表达率为31.6%,两组差异有统计学意义(P<0.05)。结论模型组大鼠SREBP-1c与JNK蛋白表达水平明显高于对照组,2型糖尿病伴肝损伤大鼠的SREBP-1c和JNK蛋白表达明显增加。Objective To explore the expression and significance of SREBP-1c and JNK in type 2 diabetic rat liver injury. Methods 2 diabetic rats with hepatic injury models were induced by high fat diet with low dose streptozotocin, the rats were fed normal diet as a control group, two groups of blood glucose levels and lipid levels were detected, the protein expression levels of JNK and SREBP-1c were detected. Results There was significant difference of the bio- chemical markers of liver function between the model group and the control group （P 〈 0.05）, it was indicated that the protein expression of SREBP-1c and JNK was increased in the liver tissue of model group, there was significant dif- ference of the protein expression of SREBP-1c and JNK between the model group and the control group （P 〈 0.05）, Conclusion The model rats group are significantly higher than the control group, SREBP-1c and JNK protein expression are significantly higher in rats with type 2 diabetes joint with liver injury.

关 键 词：2型糖尿病 固醇调节元件结合蛋白 C-JUN氨基末端激酶 肝损伤 

分 类 号：R587.1[医药卫生—内分泌]