巴戟天醇提物对大鼠肾缺血再灌注损伤的保护作用  

Protective effects of radix morindae officinalis ethanolic extracts on renal ischemia reperfusion injury in rats

作　　者：张向前[1,2] 王雪侠[3] 

机构地区：[1]郑州大学基础医学院,河南郑州450052 [2]河南电力医院检验科,河南郑州450005 [3]郑州市妇幼保健院检验科,河南郑州450012

出　　处：《中国医药导报》2013年第5期22-24,共3页China Medical Herald

摘　　要：目的探讨巴戟天醇提物对肾缺血再灌注损伤(IRI)的保护作用。方法将80只健康雄性Wistar大鼠随机均分为假手术组、IRI模型组、巴戟天高剂量组和巴戟天低剂量组4组,每组各20只。巴戟天高、低剂量组大鼠均连续灌胃给予一定浓度的巴戟天醇提物,而假手术组和IRI模型组大鼠仅给予生理盐水灌胃,用药7 d后,建立肾IRI模型。采用生物化学法检测血清中尿素氮(BUN)、肌酐(Cr)水平以及肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平,采用TUNEL法检测肾组织细胞凋亡情况。结果与IRI模型组相比,巴戟天高剂量组和巴戟天低剂量组血清BUN、Cr水平降低(P<0.05);巴戟天高剂量组和巴戟天低剂量组肾组织中SOD、GSH-Px水平升高,而MDA水平降低(P<0.05);巴戟天高剂量组和巴戟天低剂量组凋亡指数降低(P<0.05)。巴戟天高剂量组和巴戟天低剂量组血清BUN、Cr水平,肾组织中SOD、GSH-Px水平,凋亡指数比较差异均有统计学意义(均P<0.05)。结论巴戟天醇提物能够保护肾IRI后的肾组织,这可能与其具有抗氧化和抗凋亡作用有关。Objective To investigate the protective effects of radix morindae officinalis ethanolic extracts on renal is- chemia-reperfusion injury （IRI） in rats. Methods 80 healthy male Wistar rats were randomly divided into 4 groups： the sham operation group, the IRI model group, the radix morindae officinalis high dose group and the radix morindae officinalis low dose group, and each group had 20 rats. The rats in the radix morindae officinalis high dose group and the radix morindae officinalis low dose group were given with radix morindae officinalism, and the rats in the sham op- eration group and the IRI model were given with saline for 7 days. Then, the renal IRI model was established. The levels of urea nitrogen （BUN） and creatinine （Cr） in blood serum, superoxidase （SOD）, glutathione peroxidase （GSH- PX） and malondialdehyde （MDA） in renal were measured. The renal cell apoptosis were determined by TUNEL method. Results Compared with the IRI group, the levels of BUN and Cr decreased significantly in the radix morindae officinalis high dose group and the radix morindae officinalis low dose group （P 〈 0.05）; the levels of SOD and GSH- PX were increased significantly and the level of MDA decreased significantly （P 〈 0.05）; the renal cell apoptotic in- dexes decreased significantly （P 〈 0.05）. There were statistical differences in the serum levels of BUN and Cr, the lev- els of SOD, GSH-Px and MDA in the renal tissues, the cell apoptotic indexes between the radix morindae officinalis high dose group and the radix morindae officinalis low dose group （all P 〈 0.05）. Conclusion Radix morindae officinalis ethanolic extracts can protect the renal after IRI in rats, this may be related to its anti-oxidant and anti-apoptosis.

关 键 词：巴戟天醇提物 肾 缺血再灌注损伤 尿素氮 肌酐 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛 细 胞凋亡 

分 类 号：R692[医药卫生—泌尿科学]